| Full text | |
| Author(s): |
Albano, Carolina Simao
[1, 2]
;
Gomes, Anderson Moreira
[1]
;
Feltran, Georgia da Silva
[1]
;
da Costa Fernandes, Celio Junior
[1]
;
Trino, Luciana Daniele
[2]
;
Zambuzzi, Willian Fernando
[1]
;
Lisboa-Filho, Paulo Noronha
[2]
Total Authors: 7
|
| Affiliation: | [1] Sao Paulo State Univ, UNESP, Bioassays & Cell Dynam Lab, Biosci Inst, Dept Chem & Biochem, Botucatu, SP - Brazil
[2] Sao Paulo State Univ, UNESP, Adv Mat & Nanotechnol Lab, Sch Sci, Dept Phys, Bauru, SP - Brazil
Total Affiliations: 2
|
| Document type: | Journal article |
| Source: | HELIYON; v. 6, n. 7 JUL 2020. |
| Web of Science Citations: | 0 |
| Abstract | |
Background: Biofunctionalization of titanium surfaces can improve host responses, especially considering the time for osteointegration and patient recovery. This prompted us to modify titanium surfaces with alendronate and albumin and to investigate the behavior of osteoblasts on these surfaces. Methods: The biofunctionalization of titanium surfaces was characterized using classical physicochemical approaches and later used to challenge pre-osteoblast cells up to 24 h. Then their viability and molecular behavior were investigated using mitochondrial dehydrogenase activity and RTq-PCR technologies, respectively. Potential stimulus of extracellular remodeling was also investigated by zymography. Results: Our data indicates a differential behavior of cells responding to the surfaces, considering the activity of mitochondrial dehydrogenases. Molecularly, the differential expression of genes related with cell adhesion highlighted the importance of Integrin-beta 1, Fak, and Src. These 3 genes were significantly decreased in response to titanium surfaces modified with alendronate, but this behavior was reverted when alendronate was associated with albumin. Alendronate-modified surfaces promoted a significant increase on ECM remodeling, as well as culminating with greater gene activity related to the osteogenic phenotype (Runx2, Alp, Bsp). Conclusion: Altogether, our study found interesting osteogenic behavior of cells in response to alendronate and albumin surfaces, which indicates the need for in vivo analyses to better consider these surfaces before clinical trials within the biomedical field. (AU) | |
| FAPESP's process: | 17/02366-3 - Biocompatibility of functionalized TiO2 thin films |
| Grantee: | Carolina Simão Albano |
| Support Opportunities: | Scholarships in Brazil - Scientific Initiation |
| FAPESP's process: | 14/22689-3 - Microvesicle/proteins-mediated paracrine signaling among bone and endothelial cells during bone development and regeneration |
| Grantee: | Willian Fernando Zambuzzi |
| Support Opportunities: | Research Grants - Young Investigators Grants |
| FAPESP's process: | 17/15035-5 - Adsorption of bisphosphonates and proteins on TiO2 surfaces for bone implants |
| Grantee: | Paulo Noronha Lisboa Filho |
| Support Opportunities: | Regular Research Grants |