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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Characterization of the in Vitro Osteogenic Response to Submicron TiO(2 )Particles of Varying Structure and Crystallinity

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Author(s):
Tovani, Camila B. [1] ; Ferreira, Claudio R. [1] ; Simao, Ana Maria S. [1] ; Bolean, Mayte [1] ; Coppeta, Luca [2] ; Rosato, Nicola [3] ; Bottini, Massimo [4, 3] ; Ciancaglini, Pietro [1] ; Ramos, Ana Paula [1]
Total Authors: 9
Affiliation:
[1] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Quim, BR-14040901 Ribeirao Preto - Brazil
[2] Univ Roma Tor Vergata, Dept Occupat Med, I-00133 Rome - Italy
[3] Univ Roma Tor Vergata, Dept Expt Med, I-00133 Rome - Italy
[4] Sanford Burnham Prebys Med Discovery Inst, La Jolla, CA 92037 - USA
Total Affiliations: 4
Document type: Journal article
Source: ACS OMEGA; v. 5, n. 27, p. 16491-16501, JUL 14 2020.
Web of Science Citations: 0
Abstract

Titanium oxide (TiO2) nano-/microparticles have been widely used in orthopedic and dental sciences because of their excellent mechanical properties, chemical stability, and ability to promote the osseointegration of implants. However, how the structure and crystallinity of TiO2 particles may affect their osteogenic activity remains elusive. Herein, we evaluated the osteogenic response to submicron amorphous, anatase, and rutile TiO2 particles with controlled size and morphology. First, the ability of TiO2 particles to precipitate apatite was assessed in an acellular medium by using a simulated body fluid (SBF). Three days after the addition to SBF, anatase and rutile TiO2 particles induced the precipitation of aggregates of nanoparticles with a platelike morphology, typical for biomimetic apatite. Conversely, amorphous TiO2 particles induced the precipitation of particles with poor Ca/P atomic ratio only after 14 days of exposure to SBF. Next, the osteogenic response to TiO2 particles was assessed in vitro by incubating MC3T3-E1 preosteoblasts with the particles. The viability and mineralization efficiency of osteoblastic cells were maintained in the presence of all the tested TiO2 particles despite the differences in the induction of apatite precipitation in SBF by TiO2 particles with different structures. Analysis of the particles' surface charge and of the proteins adsorbed onto the particles from the culture media suggested that all the tested TiO2 particles acquired a similar biological identity in the culture media. We posited that this phenomenon attenuated potential differences in osteoblast response to amorphous, anatase, and rutile particles. Our study provides an important insight into the complex relationship between the physicochemical properties and function of TiO2 particles and sheds light on their safe use in medicine. (AU)

FAPESP's process: 14/24249-0 - Association of osteogenic proteins in biominerals and metallic oxides doped with rare-earth: interaction with membrane models systems
Grantee:Camila Bussola Tovani
Support Opportunities: Scholarships in Brazil - Doctorate (Direct)