Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Immunoinflammatory response and bioactive potential of GuttaFlow bioseal and MTA Fillapex in the rat subcutaneous tissue

Full text
Author(s):
Delfino, Mateus Machado [1] ; Guerreiro-Tanomaru, Juliane Maria [1] ; Tanomaru-Filho, Mario [1] ; Sasso-Cerri, Estela [2] ; Cerri, Paulo Sergio [2]
Total Authors: 5
Affiliation:
[1] Sao Paulo State Univ, Dept Restorat Dent, Dent Sch, UNESP, Araraquara, SP - Brazil
[2] Sao Paulo State Univ, Dept Morphol Genet Orthodont & Pediat Dent, Lab Histol & Embryol, Dent Sch, UNESP, Araraquara, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: SCIENTIFIC REPORTS; v. 10, n. 1 APR 28 2020.
Web of Science Citations: 0
Abstract

To evaluate the effect of GuttaFlow bioseal (GFB) and MTA Fillapex (MTAF) in comparison with Endofill (EF) in the subcutaneous tissue. Polyethylene tubes with GFB, MTAF, EF or empty tubes (control group; CG) were implanted into subcutaneous of rats. After 7, 15, 30 and 60 days, the capsule thickness, inflammatory reaction, interleukin-6 (IL-6), vascular endothelial growth factor (VEGF), caspase-3, TUNEL-positive cells, von Kossa and ultrastructural features were evaluated. The data were statistically analyzed (p <= 0.05). At all periods, the number of IL-6- and VEGF-immunolabelled cells, and capsule thickness were lower in GFB than MTAF, which was lower than EF (p<0.0001). At 60 days, the number of inflammatory cells was similar in GFB and MTAF (p=0.58). Significant differences in the number of TUNEL- and caspase-3-positive cells were not observed among GFB, MTAF and CG whereas the highest values were found in EF specimens. The EF specimens exhibited several cells with condensed chromatin, typical of apoptosis. von Kossa-positive and birefringent structures were only observed in GFB and MTAF, suggesting the presence of calcite crystals. Taken together, these results show that cellular and structural damage induced by GFB and MTAF sealers were recovery over time. Moreover, these sealers express bioactive potential in subcutaneous tissue. (AU)

FAPESP's process: 16/09264-9 - Inhibitory effect of Tumor Necrosis Factor-± and Interleukin-1 in the eruptive pathway of rat molars
Grantee:Paulo Sergio Cerri
Support Opportunities: Regular Research Grants