Standardized Arrabidaea chica Extract Shows Cytoprotective Effects in Zoledronic Acid-Treated Fibroblasts and Osteoblasts

Author(s)

Wiziack Zago, Patricia Maria ^[1] ; Oliveira Sousa, Ilza Maria ^[2] ; Serva-Medina, Leila ^[2] ; Jorge, Michelle Pedroza ^[2] ; Lima Neto, Lidio Goncalves ^[3] ; Hass, Viviane ^[4] ; Li, Xin ^[5] ; Gois Ruiz, Ana Lucia Tasca ^[2] ; Saxena, Deepak ^[5] ; Foglio, Mary Ann ^[2]

Affiliation

^[1] Sao Leopoldo Mandic Med Sch, Araras, SP - Brazil

^[2] Univ Estadual Campinas, Fac Pharmaceut Sci, Rua Candido Portinari 200, BR-13083871 Campinas, SP - Brazil

^[3] CEUMA Univ, Postgrad Program Parasit Biol, Sao Luis, Maranhao - Brazil

^[4] Univ Missouri, Sch Dent, Kansas City, MO - USA

^[5] NYU, Coll Dent, New York, NY - USA

• Document type: Journal article
• Source: CLINICAL COSMETIC AND INVESTIGATIONAL DENTISTRY; v. 12, p. 327-333, 2020.
• Web of Science Citations: 0

Abstract

Introduction: Osteonecrosis of the jaw is a condition associated with intraoral ulceration and bone necrosis induced by antiresorptive medications, such as zoledronic acid, a bisphosphonate. Previous data on Arrabidaea chica (H\&B.) Verlot wound healing activity prompted the study reported herein on A. chica standardized hydro alcoholic extract in vitro cytoprotective activity data on epithelial and osteoblastic cells exposed to zoledronic acid (ZA). Methods: Primary human gingival fibroblasts and murine pre-osteoblasts were treated with ZA 10 mu M together with 5 or 10 mu g.mL(-1) A. chica extract for 24h and 48 h. At both times, cells were submitted to viability assay and caspase 3/7 activation evaluation. Statistical analysis used one-way ANOVA and p=0.05. Results: In cell viability assay, a drastic damage effect of ZA appeared after 48 h in both epithelial (55.8%) and pre-osteoblastic cells (39.7%). When treated with ZA in combination with A. chica extract, cells showed higher viability values: 74.1%-82.3% for fibroblasts and 66% for pre-osteoblasts. Furthermore, the combined treatment presented lower caspase 3/7 activation in fibroblasts and pre-osteoblasts. Conclusion: At low concentrations, A. chica extract showed promising cytoprotective effects against ZA-induced damage actions; however, further in vitro and in vivo studies are required to establish the mechanism of action. (AU)

• FAPESP's process: 11/09127-8 - Arrabidaea chica Verlot: micro and nanoencapsulação of standardized crude extracts in carajiruna and the influence on wound healing of skin and mucosa
• Grantee: Mary Ann Foglio
• Support Opportunities: Regular Research Grants
• FAPESP's process: 10/07781-0 - Arrabidaea chica Verlot: in vitro evaluation of toxicity, antimicrobial and cytoprotective activities from standardized free crude extract combined with zoledronic acid, and microencapsulation studies
• Grantee: Patrícia Maria Wiziack Zago
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 11/21006-1 - Arrabidaea chica Verlot ethanolic extract activity on fibroblasts, keratinocytes, osteoblasts and osteoclasts under a bisphosphonate treatment
• Grantee: Patrícia Maria Wiziack Zago
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate