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Bisphosphonates and implantology: effect of metalloproteinases inhibitors in in vitro model

Grant number: 19/16886-4
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): March 01, 2020
Effective date (End): January 31, 2023
Field of knowledge:Health Sciences - Dentistry - Periodontology
Principal researcher:Fernanda Gonçalves Basso
Grantee:Laís Medeiros Cardoso
Home Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil

Abstract

Bisphosphonates are indicated for treatment of osteolytic diseases, aiming to control bone resorption. These drugs show high affinity to mineral tissues and are released during traumatic or inflammatory events, leading to cytotoxic and cytopatic effects. For this reason, the installation of oral osseointegrated implants is still controversial for patients ongoing bisphosphonates treatment. Besides the cytotoxic effects, bisphosphonates may also induce the overexpression of matrix metalloproteinases (MMPs), negatively interfering on osseointegration and biological sealing. Therefore, the modulation of these enzymes could favor these tissue events. The aim of this study will be to evaluate the effect of two MMPs inhibitors - proanthocyanidins and naringenin, on osteoblasts and fibroblasts seeded onto titanium surfaces and treated with Zoledronic Acid (ZA). Titanium discs will be placed in cell-culture plates and cells will be seeded onto them. After 24 hours, ZA will be applied and after 24 or 48 hours cell proliferation, adhesion, collagen synthesis, alkaline phosphatase activity, gene expression, synthesis and activity of MMPs will be evaluated. Expression of TIMP-1 and -2 will also be assessed. Results will be statistically evaluated according to data distribution. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
BASSO, FERNANDA GONCALVES; CARDOSO, LAIS MEDEIROS; RIBEIRO, ISABELA MASSARO; RIZZI, ELLEN; PANSANI, TAISA NOGUEIRA; HEBLING, JOSIMERI; DE SOUZA COSTA, CARLOS ALBERTO. Influence of bisphosphonates on oral implantology: Sodium alendronate and zoledronic acid enhance the synthesis and activity of matrix metalloproteinases by gingival fibroblasts seeded on titanium. ARCHIVES OF ORAL BIOLOGY, v. 127, JUL 2021. Web of Science Citations: 0.
CARDOSO, LAIS MEDEIROS; PANSANI, TAISA NOGUEIRA; HEBLING, JOSIMERI; DE SOUZA COSTA, CARLOS ALBERTO; BASSO, FERNANDA GONCALVES. Chemotherapy drugs and inflammatory cytokines enhance matrix metalloproteinases expression by oral mucosa cells. ARCHIVES OF ORAL BIOLOGY, v. 127, JUL 2021. Web of Science Citations: 0.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.