| Full text | |
| Author(s): |
de Lima, Juliana Maria
[1]
;
Furlani, Izadora Liranco
[1]
;
Guimaraes Silva, Larissa Ramos
[1, 2]
;
Valverde, Alessandra Leda
[2]
;
Cass, Quezia Bezerra
[1]
Total Authors: 5
|
| Affiliation: | [1] Univ Fed Sao Carlos, Dept Quim, Separare, Sao Carlos - Brazil
[2] Univ Fed Fluminense, Inst Quim, Lab Prod Nat, Niteroi, RJ - Brazil
Total Affiliations: 2
|
| Document type: | Journal article |
| Source: | ANALYTICAL METHODS; v. 12, n. 33, p. 4116-4122, SEP 7 2020. |
| Web of Science Citations: | 0 |
| Abstract | |
Functionalized micro- and nano-sized magnetic beads (MBs) have been widely used as versatile supports for proteins, enzymes, and drugs. Immobilized protein on MB surfaces has been successfully applied for ligand fishing assays allowing for direct identification of active ligands from complex mixtures, such as natural products and synthetic libraries. MBs with different properties such as different core compositions, sizes, coatings, and surface modifications are available commercially. Studies have been conducted to understand the role of these properties for ligand fishing assays. Here we evaluated, for the first time, the effect of MB size on the ligand fishing assay for acetylcholinesterase fromElectrophorus electricus(AChE). For this purpose, four commercially available amine-terminated magnetic particles with diameters ranging from 4.5 nm to 106 mu m were evaluated to fish out galantamine, a well-known AChE inhibitor, from an aqueous solution. All MBs were efficient at using glutaraldehyde to covalently immobilize AChE. The particles with diameters of about 1 mu m (small microparticles) presented a higher protein mass capacity per milligram of particle than did those with diameters of about 4.5 nm (nanoparticles) and those with diameters of about 106 mu m (large microparticles). The influence of these supports on the produced AChE-MBs with regards to hydrolysis turnover and ligand fishing was evaluated and is fully discussed. (AU) | |
| FAPESP's process: | 14/50249-8 - Green chemistry: sustainable synthetic methods employing benign solvents, safer reagents, and bio-renewable feedstock |
| Grantee: | Arlene Gonçalves Corrêa |
| Support Opportunities: | Research Grants - Research Centers in Engineering Program |
| FAPESP's process: | 18/03035-3 - Development of bioreactors for online studies of drug metabolism |
| Grantee: | Izadora Liranço Furlani |
| Support Opportunities: | Scholarships in Brazil - Doctorate (Direct) |