Study of Bacillus spp. strains used in the production of gamma-polyglutamic acid in submerged culture

Author(s)

Campos, V ^[1] ; Pimentel Filho, N. J. ^[2] ; Medeiros, T. A. M. ^[1]

Affiliation

^[1] Sao Paulo State Univ, Sci & Technol Inst Sorocaba ICTS, Sao Paulo - Brazil

^[2] Univ Fed Sao Carlos, Ctr Nat Sci, Buri, SP - Brazil

• Document type: Journal article
• Source: INTERNATIONAL FOOD RESEARCH JOURNAL; v. 27, n. 3, p. 427-434, JUN 2020.
• Web of Science Citations: 0

Abstract

Gamma-polyglutamic (gamma-polyglutamic) acid has received considerable attention for environmental applications. gamma-polyglutamic acid can be produced by fermenting soybeans and by submerged culture using the bacterium Bacillus subtilis. Although it is practically impossible to chemically synthesise gamma-polyglutamic acid, this biopolymer can be obtained through microbial culture. The microorganism most commonly used in the production of gamma-polyglutamic acid, among other strains, is B. subtilis, which has been investigated for the production of biopolymers. The purpose of the present work was to evaluate the production of gamma-polyglutamic acid from submerged culture of different Bacillus strains. The evaluation of the efficiency of Bacillus strains in producing gamma-polyglutamic acid indicated that B. licheniformis produced a higher volume of the biopolymer than B. subtilis. The highest gamma-polyglutamic acid production by B. licheniformis was 398.1 mg L-1. B. licheniformis was found to be an L-glutamic acid-independent bacterium for the production of gamma-polyglutamic acid. B. licheniformis and B. subtilis growth and gamma-polyglutamic acid production rates were also influenced by the culture media. (c) All Rights Reserved (AU)

• FAPESP's process: 15/02650-8 - Production and environmental application of y-polyglutamic acid by cultivation of B. subtilis in soy substrate
• Grantee: Valquiria de Campos
• Support Opportunities: Regular Research Grants