| Full text | |
| Author(s): |
Gelain, Lucas
[1, 2]
;
Pabst, Martin
[1]
;
da Cruz Pradella, Jose Geraldo
[3]
;
da Costa, Aline Carvalho
[2]
;
van der Wielen, Luuk
[1, 4]
;
van Gulik, Walter M.
[1]
Total Authors: 6
|
| Affiliation: | [1] Delft Univ Technol, Dept Biotechnol, Van der Maasweg 9, NL-2629 HZ Delft - Netherlands
[2] Univ Estadual Campinas, Dev Proc & Prod, Av Albert Einstein 500, Campinas - Brazil
[3] Univ Fed Sao Paulo, Inst Sci & Technol, Av Cesare Mansueto Giulio Lattes 1201, Sao Jose Dos Campos - Brazil
[4] Univ Limerick, Bernal Inst, Limerick V94 T9PX - Ireland
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | JOURNAL OF PROTEOMICS; v. 227, SEP 15 2020. |
| Web of Science Citations: | 2 |
| Abstract | |
The wild type strain Trichoderma harzianum was able to synthesize enzymes that can catalyse the hydrolysis of p-nitrophenyl-beta-D-glucopyranoside (PNPGase) in glucose-limited chemostat cultures. Fructose/glucose and sucrose conditions provided low levels of PNPGase activity. To investigate whether under these conditions other enzymes were produced, a shotgun proteomics analysis of their supernatants was performed. The analysis has indicated that the different carbon sources used influenced the amounts of proteins secreted including 1,3-beta-glucanosyltransferase, alpha-1,2-mannosidase, alpha-galactosidase and glucan 1,3-beta-glucosidase. The analysis has also suggested the presence of beta-glucosidase, which could also be represented by PNPGase activity. Intracellular metabolites were quantified during PNPGase production for the condition using 20 g/L of glucose in the feed and differences were observed, indicating that intracellular glucose could be inhibiting PNPGase production. Significance: This work shows that sugars such as glucose, fructose/glucose and sucrose can be used as substrates for the continuous synthesis of different enzymes under carbon-limited conditions by Trichoderma harzianum. As far as we know, this is the first work about the continuous synthesis of enzymes under carbon-limited conditions suggesting that different easily assimilated carbon sources can be used to generate different enzymatic cocktails. Each enzyme or uncharacterized protein suggested by shotgun proteomics has the potential to become a promising product for biotechnological applications. (AU) | |
| FAPESP's process: | 14/22537-9 - Mathematical modeling of enzymes production by Trichoderma harzianum in submerged fermentation |
| Grantee: | Lucas Gelain |
| Support Opportunities: | Scholarships in Brazil - Doctorate |