Brazilian southeast brown propolis: gas chromatography method development for its volatile oil analysis, its antimicrobial and leishmanicidal activities evaluation

Author(s)

Ribeiro, Victor Pena ^[1] ; Arruda, Caroline ^[1] ; Mejia, Jennyfer Andrea Aldana ^[1] ; Candido, Ana Carolina Bolela Bovo ^[2] ; dos Santos, Raquel Alves ^[2] ; Magalhaes, Lizandra Guidi ^[2] ; Bastos, Jairo Kenupp ^[1]

Affiliation

^[1] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Ave Cafe, BR-14040930 Ribeirao Preto, SP - Brazil

^[2] Univ Franca, Ctr Res Sci & Technol, Res Grp Nat Prod, Franca, SP - Brazil

• Document type: Journal article
• Source: Phytochemical Analysis; v. 32, n. 3 SEP 2020.
• Web of Science Citations: 2

Abstract

Introduction Propolis is widely used in folk medicine, and many factors can affect its chemical composition, including abiotic factors that can influence plants and bees. Therefore, analytical methods are powerful techniques in the quality control of such products. Objective Develop and validate an analytical method for quantifying volatile compounds in Brazilian brown propolis, and evaluate its biological activities. Methods A gas chromatography flame ionisation detector (GC-FID) analytical method was validated, attending the parameters of international validation guidelines as ANVISA 2017 and ICH 2005, for quantification of compounds present in volatile oils from propolis. Evaluation of cytotoxic, antimicrobial, and leishmanicidal activities of the oil. Results The compounds 1,8-cineole, terpinen-4-ol,alpha-copaene,beta-caryophyllene,gamma-muurolene, nerolidol, spathulenol, and gamma-palmitolactone were isolated from the volatile fraction of a Brazilian brown propolis and used in the method validation. All the validation parameters of the method were satisfactory. The volatile fraction displayed a significant leishmanicidal activity, with half maximal inhibition concentration (IC50) = 21.3 mu g/mL against amastigote forms and IC50= 25.1 mu g/mL against promastigote forms ofLeishmania amazonensis.The oil also displayed an antibacterial effect by inhibiting the growth ofStreptococcus mutansandStaphylococcus aureusat 25 mu g/mL and 50 mu g/mL, respectively, but it was not cytotoxic against AGP-01, He-La and CHO-K1cell lines, with IC50> 100 mu g/mL. Conclusion The GC-FID method can be a useful tool in the quality control of propolis material. The southeast brown propolis showed a high chemical complexity in its volatile fraction, which displayed leishmanicidal activity and bactericidal activity. (AU)

• FAPESP's process: 17/04138-8 - Attainment of chemical, analytical, biological, pharmacological and technological studies to fill the gaps on the development of Brazilian propolis sector
• Grantee: Jairo Kenupp Bastos
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 17/08386-6 - Phytochemical study, development of analytical method and biological evaluation of brown propolis produced by Apis mellifera from Morus Alba
• Grantee: Victor Pena Ribeiro
• Support Opportunities: Scholarships in Brazil - Doctorate