SIRT1-dependent effects of resveratrol and grape juice in an in vitro model of preeclampsia

Author(s)

Viana-Mattioli, Sarah ^[1] ; Cinegaglia, Naiara ^[1] ; Bertozzi-Matheus, Mariana ^[1] ; Bueno-Pereira, Thaina Omia ^[1] ; Caldeira-Dias, Mayara ^[1] ; Cavalli, Ricardo Carvalho ^[2] ; Sandrim, Valeria Cristina ^[1]

Affiliation

^[1] Univ Estadual Paulista, UNESP, Inst Biosci Botucatu, Dept Biophys & Pharmacol, BR-18680000 Botucatu, SP - Brazil

^[2] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Gynecol & Obstet, BR-14049900 Ribeirao Preto, SP - Brazil

• Document type: Journal article
• Source: BIOMEDICINE & PHARMACOTHERAPY; v. 131, NOV 2020.
• Web of Science Citations: 1

Abstract

Preeclampsia (PE) is a multifactorial hypertensive disorder of pregnancy that is partly responsible for both maternal and fetal morbidity and mortality levels worldwide. It has been recently discovered that sirtuin-1 (SIRT1) is reduced in the circulation and in an in vitro model of PE. Therefore, in this study, we investigated the effects of trans-resveratrol, a potent antioxidant and activator of SIRT1, on oxidative stress and nitric oxide (NO) production in an in vitro model of PE compared to gestational hypertensive (GH) and healthy pregnant (HP) women. Furthermore, we also evaluated the effects of an acute intake of grape juice on women with PE to assess whether it could mimic in vitro trans-resveratrol supplementation. (1) In the GH group, resveratrol decreased intracellular reactive oxygen species (ROS) and increased their antioxidant capacity, while inhibiting SIRT1 reestablished previous levels. (2) In PE, inhibition of SIRT1 increased antioxidant activity. (3) Intracellular NO and supernatant nitrite levels were increased by inhibiting SIRT1 in the PE group. (4) Grape juice intake increased intracellular NO levels versus before grape juice intake control; however, the inhibition of SIRT1 before grape juice intake initially increased NO, but decreased it 1 h after grape juice intake. In conclusion, activating SIRT1 by using resveratrol alone may not be beneficial to women with PE, and GH and PE seem to have different responsive mechanisms to this molecule. Furthermore, grape juice intake seems to have different effects compared to resveratrol supplementation alone in this in vitro model of PE, demonstrating the potential of the combination of other biologically active molecules from grape juice over the SIRT1-eNOS-NO in PE treatment. (AU)

• FAPESP's process: 19/06118-0 - Analysis and the expression of Sirt1 and its modulation for resveratrol in vitro model of Pré-Eclampsia
• Grantee: Sarah Viana Mattioli
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 19/07230-8 - Arginase in preeclampsia: study of genetic polymorphism, circulating factors and in vitro assays
• Grantee: Valeria Cristina Sandrim
• Support Opportunities: Regular Research Grants
• FAPESP's process: 15/20461-8 - Role of NRF2 and HO-1 in preeclampsia: polymorphisms, circulating factors and in vitro assays
• Grantee: Valeria Cristina Sandrim
• Support Opportunities: Regular Research Grants