A simple method for purification of Strongyloides venezuelensis eggs from rat faeces

Author(s)

Roldan, W. H. ^{[1, 2]} ; Martins de Paula, F. ^{[1, 2]} ; Gryschek, R. C. B. ^{[1, 2]}

Affiliation

^[1] Univ Sao Paulo, Inst Med Trop Sao Paulo, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Fac Med, Hosp Clin, Lab Imunopatol Esquistossomose, Lab Invest Med LIM, Sao Paulo - Brazil

• Document type: Journal article
• Source: JOURNAL OF HELMINTHOLOGY; v. 94, NOV 18 2020.
• Web of Science Citations: 0

Abstract

The aim of this study was to develop a simple method to purify Strongyloides eggs from rat faeces using a sucrose gradient centrifugal-flotation technique. This procedure is simple, rapid and possesses a high efficiency in recovering Strongyloides eggs without faecal detritus in less than one hour, thus eliminating the use of complex apparatus and different chemical substances. The possibility of working with pure and live Strongyloides eggs opens up a wide range of future studies on the biology of this parasite. This study constitutes the first report in the scientific literature on purifying Strongyloides eggs using a sucrose density gradient. (AU)

• FAPESP's process: 16/06185-0 - Proteomic analysis of evolutionary stages from Strongyloides venezuelensis
• Grantee: Fabiana Martins de Paula
• Support Opportunities: Regular Research Grants