Antibacterial activities and antiproliferative assays over a tumor cells panel of a silver complex with 4-aminobenzoic acid: Studies in vitro of sustained release using bacterial cellulose membranes as support

Author(s):Show less -

Simei Aquaroni, Nayara Ap. ^[1] ; Nakahata, Douglas H. ^[1] ; Lazarini, Silmara C. ^[1] ; Resende, Flavia A. ^[1] ; Candido, Amanda L. P. ^[1] ; Barud, Hernane da Silva ^[1] ; Claro, Amanda Maria ^[1] ; de Carvalho, Joao Ernesto ^[2] ; Ribeiro, Camila M. ^[3] ; Pavan, Fernando R. ^[3] ; Lustri, Bruna C. ^[3] ; Machado Ribeiro, Tamara Renata ^[3] ; Moreira, Cristiano G. ^[3] ; Candido, Tuany Zambroti ^[4] ; Passos Lima, Carmen Silvia ^[4] ; Ruiz, Ana Lucia T. G. ^[2] ; Corbi, Pedro P. ^[5] ; Lustri, Wilton R. ^[1]

Affiliation

^[1] Univ Araraquara UNIARA, BR-14801320 Araraquara, SP - Brazil

^[2] Univ Campinas UNICAMP, Fac Pharmaceut Sci, BR-13081970 Campinas, SP - Brazil

^[3] Sao Paulo State Univ UNESP, Sch Pharmaceut Sci, BR-14801903 Araraquara, SP - Brazil

^[4] Univ Campinas UNICAMP, Fac Med Sci, BR-13081970 Campinas, SP - Brazil

^[5] Univ Campinas UNICAMP, Inst Chem, BR-13083970 Campinas, SP - Brazil

• Document type: Journal article
• Source: Journal of Inorganic Biochemistry; v. 212, NOV 2020.
• Web of Science Citations: 0

Abstract

The aims of this work were to evaluate the antibacterial and antiproliferative potential in vitro of the metal complex with 4-aminobenzoic acid (Ag-pABA) and a drug delivery system based on bacterial cellulose (BC-Ag-pABA). The Ag-pABA complex was characterized by elemental analysis, high resolution mass spectrometry and single-crystal X-ray diffraction techniques, which indicated a 1:2 metal/pABA composition plus a nitrate ion coordinated to silver by the oxygen atom, with the coordination formula {[}Ag (C7H7NO2)(2)(NO3)]. The coordination of pABA to the silver ion occurred by the nitrogen atom. The in vitro antibacterial activity of the complex evaluated by minimum inhibitory concentration assays demonstrated the effective growth inhibitory activity against Gram-positive, Gram-negative biofilm producers and acid-alcohol resistant Bacillus. The anti proliferative activities against a panel of eight tumor cells demonstrated the activity of the complex with a significant selectivity index (SI). The DNA interaction capacity and the Ames Test indicated the absence of mutagenicity. The BC-Ag-pABA composite showed an effective capacity of sustained release of Ag-pABA. The observed results validate further studies on its mechanisms of action and the conditions that mediate the in vivo biological effects using animal models to confirm its safety and effectiveness for treatment of skin and soft tissues infected by bacterial pathogens, urinary tract infections and cancer. (AU)

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• Grantee: Bruna Cardinali Lustri
• Support Opportunities: Scholarships in Brazil - Master
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• Grantee: Wilton Rogério Lustri
• Support Opportunities: Regular Research Grants
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• Grantee: Wilton Rogério Lustri
• Support Opportunities: Regular Research Grants
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• Grantee: Hernane da Silva Barud
• Support Opportunities: Regular Research Grants
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• Grantee: Cristiano Gallina Moreira
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• Grantee: Camila Maríngolo Ribeiro
• Support Opportunities: Scholarships in Brazil - Doctorate
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