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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Infraphysiological 17 beta-estradiol (E2) concentration compromises osteoblast differentiation through Src stimulation of cell proliferation and ECM remodeling stimulus

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Author(s):
Barneze Costa, Sarah Maria [1] ; Feltran, Georgia Silva [2] ; Namba, Vickeline [1] ; Silva, Tabata Marilda [1] ; Hallur, Raghavendra Lakshmana Shetty [1, 3] ; Saraiva, Patricia Pinto [1] ; Zambuzzi, Willian Fernando [2] ; Nogueira, Celia Regina [1]
Total Authors: 8
Affiliation:
[1] Sao Paulo State Univ UNESP, Sch Med Botucatu FMB, Expt Res Unit, BR-18618970 Botucatu, SP - Brazil
[2] Sao Paulo State Univ UNESP, Biosci Inst, Dept Chem & Biochem, BR-18618970 Botucatu, SP - Brazil
[3] Sao Paulo State Univ UNESP, Sch Med Botucatu FMB, Dept Gynecol & Obstet, BR-18618970 Botucatu, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Molecular and Cellular Endocrinology; v. 518, DEC 1 2020.
Web of Science Citations: 0
Abstract

It has been shown that 17 beta-estradiol (E2) helps to prevent bone loss. This study was undertaken to verify whether E2 action in human osteoblasts involves changes in the transcriptional profile of the TNF-alpha, IFN-gamma, NF-kappa B, TRAIL, TGF-beta, MMP2, MMP9, RECK, TIMP1, TIMP2, CDK2, CDK4, SRC, RUNX2, and SHH genes. Infraphysiological doses of E2 elevated mRNAs in all genes except for INF-gamma, TRAIL, and TGF-beta. Importantly, a significant increase in the CDKs -2 and -4 genes was found, which strongly suggests cell cycle progression, with a potential dependency of Src involvement, as well as a suppression of the osteoblast differentiation machinery, with ECM remodeling being involved. These data suggest that E2 plays an important role in bone formation and remodeling, and Src seems to play a pivotal role in driving cell proliferation and ECM remodeling. Taken together, these findings contribute to an understanding of the effects of infraphysiological E2 on modulating bone homeostasis, favoring bone resorption, and leading to osteoporosis. (AU)

FAPESP's process: 14/15529-0 - Effect of the estrogen and thyroid hormone on gene and protein expression of RANKL and TNF-± in osteoblastic cells derived from the adipose tissue
Grantee:Sarah Maria Barneze Costa
Support Opportunities: Scholarships in Brazil - Master
FAPESP's process: 14/22689-3 - Microvesicle/proteins-mediated paracrine signaling among bone and endothelial cells during bone development and regeneration
Grantee:Willian Fernando Zambuzzi
Support Opportunities: Research Grants - Young Investigators Grants
FAPESP's process: 14/16406-9 - EVALUATION OF ACTION ESTROGEN AND THYROID HORMONE ACTION UPON NONCODING RNA EXPRESSION IN OSTEOBLASTIC CELLS DERIVED OF ADIPOSE TISSUE
Grantee:Celia Regina Nogueira
Support Opportunities: Regular Research Grants