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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Interaction between Lactobacillus reuteri and periodontopathogenic bacteria using in vitro and in vivo (G. mellonella) approaches

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Author(s):
Santos, Thais Aguiar [1] ; Scorzoni, Liliana [1] ; Correia, Raquel [1] ; Junqueira, Juliana Campos [1] ; Anbinder, Ana Lia [1]
Total Authors: 5
Affiliation:
[1] Sao Paulo State Univ UNESP, Inst Sci & Technol, Av Engenheiro Francisco Jose Longo 777, BR-12245000 Sao Jose Dos Campos, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: PATHOGENS AND DISEASE; v. 78, n. 8 DEC 2020.
Web of Science Citations: 5
Abstract

Periodontitis is a multifactorial inflammatory disease, and the major cause of tooth loss in adults. New therapies have been proposed for its treatment, including the use of probiotics such as Lactobacillus reuteri. The objective of this study was to evaluate the antimicrobial effects of L. reuteri: live, heat-killed and culture filtrate (cell-free supernatant), on periodontopathogenic bacteria (Fusobacterium nucleatum and Aggregatibacter actinomycetemcomitans) in vitro, as well as the in vivo survival curve, hemocyte density and microbial recovery using Galleria mellonella. For in vitro assays, all preparations reduced colony forming units of F. nucleatum, while only live L. reuteri reduced the growth of A. actinomycetemcomitans. All treatments reduced periodontopathogenic bacteria growth in vivo. The treatment with the supernatant increased the survival of larvae infected with F. nucleatum more than the treatment with live L. reuteri, and none of the treatments altered the survival of A. actinomycetemcomitans-infected larvae. In addition, the treatment with L. reuteri preparations did not alter the hemocyte count of F. nucleatum- and A. actinomycetemcomitans-infected larvae. This study demonstrated that L. reuteri preparations exerted antimicrobial effects and increased the survival of G. mellonella infected by F. nucleatum, although only live L. reuteri was able to reduce the growth of A. actinomycetemcomitans in vitro. (AU)

FAPESP's process: 16/06946-1 - EFFECTS OF VIABLE, NON-VIABLE Lactobacillus reuteri AND SUPERNATANT ON PERIODONTAL DISEASE: AN in vitro AND in vivo STUDY
Grantee:Ana Lia Anbinder
Support Opportunities: Regular Research Grants
FAPESP's process: 16/25795-4 - Paraprobiotic effect of Lactobacillus reuteri on Prevotella intermedia infection in an invertebrate model
Grantee:Raquel Correia
Support Opportunities: Scholarships in Brazil - Scientific Initiation