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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Prevalence of Enteropathogens and Virulence Traits in Brazilian Children With and Without Diarrhea

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Author(s):
Merino, Victor R. [1] ; Nakano, Viviane [1, 2] ; Delannoy, Sabine [3] ; Fach, Patrick [3] ; Alberca, Gabriela G. F. [1] ; Farfan, Mauricio J. [4] ; Piazza, Roxane M. F. [5] ; Avila-Campos, Mario J. [1]
Total Authors: 8
Affiliation:
[1] Univ Sao Paulo, Inst Ciencias Biomed, Dept Microbiol, Lab Anaerobios, Sao Paulo - Brazil
[2] GeneOne, DASA, Sao Paulo - Brazil
[3] Univ Paris Est, Agence Natl Secur Sanit Alimentat Environm & Trav, Food Safety Lab, Champs Sur Marne - France
[4] Univ Chile, Hosp Dr Luis Calvo Mackenna, Fac Med, Dept Pediat & Cirugia Infantil, Campus Oriente, Santiago - Chile
[5] Inst Butantan, Lab Bacteriol, Sao Paulo - Brazil
Total Affiliations: 5
Document type: Journal article
Source: FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY; v. 10, SEP 25 2020.
Web of Science Citations: 0
Abstract

The use of molecular diagnostics for pathogen detection in epidemiological studies have allowed us to get a wider view of the pathogens associated with diarrhea, but the presence of enteropathogens in asymptomatic individuals has raised several challenges in understanding the etiology of diarrhea, and the use of these platforms in clinical diagnosis as well. To characterize the presence of the most relevant bacterial enteropathogens in diarrheal episodes, we evaluated here the prevalence of diarrheagenicE. colipathotypes,Salmonellaspp., andYersinia enterocoliticain stool samples of children with and without diarrhea using real-time quantitative PCR (qPCR). We found that the presence of genetic markers associated with bacterial pathogens was significantly higher in stool samples from the diarrhea group compared to the control (P< 0.001). Bacterial loads in samples positive foreaeandaggRmarkers were also determined. Compared to samples from asymptomatic children, a significantly higher number of copies of theeaegene were found in diarrhea samples. Also, the presence of genetic markers associated with STEC strains with clinical significance was evaluated ineae-positive samples by high-throughput real-time PCR. The data presented herein demonstrated that asymptomatic children of an urban area in Brazil might be enteropathogen reservoirs, especially for STEC. (AU)

FAPESP's process: 17/14821-7 - Exploring novel virulence strategies in Escherichia coli
Grantee:Tânia Aparecida Tardelli Gomes do Amaral
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 13/17739-9 - Clonal evaluation of Clostridium perfringens strains isolated from humans and animals by Multilocus sequence typing (MLST)
Grantee:Mario Júlio Ávila-Campos
Support Opportunities: Regular Research Grants