Inverse sex-based expression profiles of PTEN and Klotho in mice

Author(s)

de Mello, Natalia Prudente ^[1] ; Andreotti, Diana Zukas ^{[2, 1]} ; Orellana, Ana Maria ^[2] ; Scavone, Cristoforo ^[2] ; Kawamoto, Elisa Mitiko ^[1]

Affiliation

^[1] Univ Sao Paulo, Lab Mol & Funct Neurobiol, Dept Pharmacol, Inst Biomed Sci 1, Ave Prof Lineu Prestes 1524, BR-05508000 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Inst Biomed Sci, Dept Pharmacol, Lab Mol Neuropharmacol, Sao Paulo - Brazil

• Document type: Journal article
• Source: SCIENTIFIC REPORTS; v. 10, n. 1 NOV 19 2020.
• Web of Science Citations: 0

Abstract

Sex differences are considered predictive factors in the development of several neurological diseases, which are also known to coincide with impaired phosphoinositide 3-kinase (PI3K)-AKT pathway activity, an essential signaling cascade involved in the control of several cellular functions such as autophagy and apoptosis. Here, under physiological conditions, we show important sex differences in the underlying balancing mechanisms that lead to similar AKT activity levels and autophagy and apoptosis processes in the two sexes. We demonstrate inverse sex-based expression of PTEN and Klotho, two important proteins that are known to negatively regulate the AKT pathway, and inverse sex-dependent levels of mTOR and FoxO3a activity. Taken together, our findings indicate that inverse sex-based regulation may be one of the underlying balancing mechanisms that differ between the sexes and a possible cause of sex-based autophagic and apoptotic responses to triggering situations that can lead to a sex-based predisposition to some neurological diseases. (AU)

• FAPESP's process: 11/21308-8 - Evaluation of PTEN compartmentalization on neurogenesis and synaptic plasticity influenced by environmental factors
• Grantee: Elisa Mitiko Kawamoto Iwashe
• Support Opportunities: Research Grants - Young Investigators Grants
• FAPESP's process: 16/07427-8 - Aging and neuroprotection: effects of Klotho protein in energetic metabolism, Na,K-ATPase signaling and adaptative response in central nervous system
• Grantee: Cristoforo Scavone
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 16/22996-9 - Evaluation of physical exercise effects on cognition of neuronal-PTEN deleted animals
• Grantee: Elisa Mitiko Kawamoto Iwashe
• Support Opportunities: Regular Research Grants
• FAPESP's process: 15/25491-2 - Evaluation of the effects of the Klotho protein on the neuronal morphological alteration characteristics of the lack of PTEN protein
• Grantee: Natalia Prudente de Mello
• Support Opportunities: Scholarships in Brazil - Master