Candidemia Candida albicans clusters have higher tendency to form biofilms than singleton genotypes

Author(s):Show less -

Diaz-Garcia, Judith ^{[1, 2]} ; Arendrup, Maiken C. ^{[3, 4, 5]} ; Canton, Rafael ^{[6, 7, 8]} ; Garcia-Rodriguez, Julio ^[9] ; Gomez, Ana ^{[1, 2]} ; Gomez, Elia ^{[6, 7, 8]} ; Orden, Beatriz ^[10] ; Parisi, Gabriella ^[11] ; Peman, Javier ^{[12, 13]} ; Posteraro, Brunella ^[14] ; Sanguinetti, Maurizio ^[15] ; Archimedes Da Matta, Daniel ^[16] ; Colombo, Arnaldo L. ^[16] ; Munoz, Patricia ^{[1, 17, 2, 18]} ; Sanchez-Carrillo, Carlos ^{[1, 17, 2]} ; Guinea, Jesus ^{[1, 17, 2]} ; Escribano, Pilar ^{[1, 2]}

Affiliation:Show less -

^[1] Hosp Gen Univ Gregorio Maranon, Clin Microbiol & Infect Dis, Madrid - Spain

^[2] Inst Invest Sanitaria Gregorio Maranon, Madrid - Spain

^[3] Univ Copenhagen, Dept Clin Med, Copenhagen - Denmark

^[4] Statens Serum Inst, Unit Mycol, Copenhagen - Denmark

^[5] Rigshosp, Dept Clin Microbiol, Copenhagen - Denmark

^[6] Hosp Ramon & Cajal, Serv Microbiol, Madrid - Spain

^[7] Inst Ramon y Cajal Invest Sanitaria IRYCIS, Madrid - Spain

^[8] Inst Salud Carlos III, Red Espanola Invest Patol Infecciosa REIPI, Madrid - Spain

^[9] Hosp La Paz, Clin Microbiol Dept, Madrid - Spain

^[10] Hosp Puerta del Hierro Majadahonda, Madrid - Spain

^[11] Azienda Osped San Camillo Forlanini, Rome - Italy

^[12] Hosp Univ & Politecn La Fe, Valencia - Spain

^[13] Inst Invest Sanitaria La Fe, Valencia - Spain

^[14] Fdn Policlin Univ A Gemelli IRCCS, Dipartimento Sci Gastroenterol Endocrinometab & N, Rome - Italy

^[15] Fdn Policlin Univ A Gemelli IRCCS, Dipartimento Sci Lab & Infettivol, Rome - Italy

^[16] Univ Fed Sao Paulo, Escola Paulista Med, Sao Paulo - Brazil

^[17] CIBER Enfermedades Resp CIBERES CB06 06 0058, Madrid - Spain

^[18] Univ Complutense Madrid, Fac Med, Med Dept, Madrid - Spain

• Document type: Journal article
• Source: Medical Mycology; v. 58, n. 7, p. 887-895, OCT 2020.
• Web of Science Citations: 0

Abstract

The capacity of Candida spp. to form biofilms allows them to attach either to living or inert surfaces, promoting their persistence in hospital environments. In a previous study, we reported strain-to-strain variations in Candida spp. biofilm development, suggesting that some genotypes may be greater biofilm formers than others. In this study, we hypothesize that isolates pertaining to clusters may be found more frequently in the environment due to their ability to form biofilms compared to singleton genotypes. Two hundred and thirty-nine Candida spp. isolates (78 clusters) from candidemia patients admitted to 16 hospitals located in different cities and countries-and the same number of singleton genotypes used as controls-were tested in terms of biofilm formation using the crystal violet and the XTT reduction assays. Candida albicans clusters showed higher biofilm formation in comparison to singleton genotypes (P < .01). The biofilms formed by intra-hospital C. albicans clusters showed higher metabolic activity (P < .05). Furthermore, marked variability was found among species and type of cluster. We observed that the higher the number of isolates, the higher the variability of biofilm production by isolates within the cluster, suggesting that the production of biofilm by isolates of the same genotype is quite diverse and does not depend on the type of cluster studied. In conclusion, candidemia Candida spp. clusters-particularly in the case of C. albicans-show significantly more biomass production and metabolic activity than singleton genotypes. (AU)

• FAPESP's process: 17/02203-7 - Multidisciplinary and international network to characterize microbiological aspects and natural history of invasive fungal infections due Candida spp
• Grantee: Arnaldo Lopes Colombo
• Support Opportunities: Research Projects - Thematic Grants