| Full text | |
| Author(s): Show less - |
Rocha, Andrea Livia
[1, 2, 3, 4]
;
de Lima, Tanes Imamura
[5, 6, 7]
;
de Souza, Gerson Profeta
[1, 2, 6]
;
Correa, Renan Oliveira
[8, 2]
;
Ferrucci, Danilo Lopes
[5, 1, 9]
;
Rodrigues, Bruno
[10]
;
Lopes-Ramos, Camila
[11, 12]
;
Nilsson, Daniel
[13, 14]
;
Knittel, Thiago Leite
[1, 2, 6]
;
Castro, Pollyana Ribeiro
[8, 2]
;
Fernandes, Mariane Font
[8, 2]
;
Martins, Flaviano Dos Santos
[15]
;
Parmigiani, Raphael Bessa
[11]
;
Silveira, Leonardo Reis
[5, 6]
;
Carvalho, Hernandes F.
[5, 9]
;
Auwerx, Johan
[7]
;
Vinolo, Marco Aurelio R.
[8, 2, 6, 16]
;
Boucher, Jeremie
[13, 14, 17]
;
Mori, Marcelo A.
[1, 2, 3, 4, 6, 16]
Total Authors: 19
|
| Affiliation: Show less - | [1] Univ Estadual Campinas, Inst Biol, Dept Biochem & Tissue Biol, Campinas - Brazil
[2] Univ Estadual Campinas, Inst Biol, Program Genet & Mol Biol, Campinas - Brazil
[3] Univ Fed Sao Paulo, Sao Paulo Sch Med, Dept Biophys, Sao Paulo - Brazil
[4] Univ Fed Sao Paulo, Program Biotechnol, Sao Paulo - Brazil
[5] Univ Estadual Campinas, Inst Biol, Dept Struct & Funct Biol, Campinas - Brazil
[6] Univ Estadual Campinas, Obes & Comorbid Res Ctr OCRC, Campinas - Brazil
[7] Ecole Polytech Fed Lausanne, Lab Integrat Syst Physiol, Interfac Inst Bioengn, Lausanne - Switzerland
[8] Univ Estadual Campinas, Inst Biol, Dept Genet Evolut Microbiol & Immunol, Campinas - Brazil
[9] Univ Estadual Campinas, Natl Inst Sci & Technol Photon Appl Cell Biol Inf, Campinas - Brazil
[10] Univ Estadual Campinas, Sch Phys Educ, Dept Adapted Phys Act, Campinas - Brazil
[11] Sirio Libanes Hosp, Ctr Mol Oncol, Sao Paulo - Brazil
[12] Harvard TH Chan Sch Publ Hlth, Dept Biostat, Boston, MA - USA
[13] Univ Gothenburg, Wallenberg Ctr Mol & Translat Med, Gothenburg - Sweden
[14] Univ Gothenburg, Lundberg Lab Diabet Res, Gothenburg - Sweden
[15] Univ Fed Minas Gerais, Inst Biol Sci, Dept Microbiol, Lab Biotherapeut Agents, Belo Horizonte, MG - Brazil
[16] Expt Med Res Cluster EMRC, Campinas - Brazil
[17] AstraZeneca, Biosci Metab Res & Early Dev Cardiovasc Renal & M, BioPharmaceut R&D, Gothenburg - Sweden
Total Affiliations: 17
|
| Document type: | Journal article |
| Source: | SCIENCE ADVANCES; v. 6, n. 49 DEC 2020. |
| Web of Science Citations: | 0 |
| Abstract | |
MicroRNAs (miRNAs) have been implicated in oxidative metabolism and brown/beige adipocyte identity. Here, we tested whether widespread changes in miRNA expression promoted by treatment with the small-molecule enoxacin cause browning and prevent obesity. Enoxacin mitigated diet-induced obesity in mice, and this was associated with increased energy expenditure. Consistently, subcutaneous white and brown adipose tissues and skeletal muscle of enoxacin-treated mice had higher levels of markers associated with thermogenesis and oxidative metabolism. These effects were cell autonomous since they were recapitulated in vitro in m urine and human cell models. In preadipocytes, enoxacin led to a reduction of miR-34a-5p expression and up-regulation of its target genes (e.g., Fgfr1, Klb, and Sirt1), thus increasing FGF21 signaling and promoting beige adipogenesis. Our data demonstrate that enoxacin counteracts obesity by promoting thermogenic signaling and inducing oxidative metabolism in adipose tissue and skeletal muscle in a mechanism that involves, at least in part, miRNA-mediated regulation. (AU) | |
| FAPESP's process: | 10/52557-0 - Identification of mechanisms responsible for beneficial effects of calorie restriction |
| Grantee: | Marcelo Alves da Silva Mori |
| Support Opportunities: | Research Grants - Young Investigators Grants |
| FAPESP's process: | 17/01184-9 - CAMeLEOm: cross-species analysis of metabolic, lifespan effects and omics of dietary restriction mimetics |
| Grantee: | Marcelo Alves da Silva Mori |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 18/15313-8 - Investigation of the molecular mechanisms involved in the interaction between microbiota-derived metabolites and host cells during inflammation |
| Grantee: | Marco Aurélio Ramirez Vinolo |
| Support Opportunities: | Research Grants - Young Investigators Grants - Phase 2 |
| FAPESP's process: | 16/23142-3 - Interaction between HIF-1 and short-chain fatty acids in the intestine: what is the role of HIF-1 acetylation? |
| Grantee: | Renan Oliveira Corrêa |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 17/07975-8 - Exploring the role of adipose tissue-derived circulating miRNAs in the beneficial effects of exercise training |
| Grantee: | Marcelo Alves da Silva Mori |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 16/24163-4 - Screening and characterization of fluoroquinolone compounds and their influence on the biogenesis of miRNAs |
| Grantee: | Gerson Profeta de Souza |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 15/01316-7 - Dicer, miRNAs and the control of mitochondrial function in the context of aging and caloric restriction |
| Grantee: | Marcelo Alves da Silva Mori |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 16/12294-7 - Dicer signalling in adipocytes controlling immune function and activation of beige cells in adipose tissue |
| Grantee: | Andréa Livia Silva Rocha |
| Support Opportunities: | Scholarships in Brazil - Doctorate |