Biochemical and morphological biomarker responses in the gills of a Neotropical fish exposed to a new flavonoid metal-insecticide

Author(s)

de Castro Sachi, Ivelise Teresa ^{[1, 2]} ; Bonomo, Marina Marques ^{[1, 2]} ; Sakuragui, Marise Margareth ^{[1, 2]} ; Modena, Pamela Zaganin ^[2] ; Paulino, Marcelo Gustavo ^[3] ; Carlos, Rose Maria ^[4] ; Fernandes, Joao Batista ^[4] ; Fernandes, Marisa Narciso ^{[1, 2]}

Affiliation

^[1] Univ Fed Sao Carlos, Postgrad Program Ecol & Nat Resources, Rodovia Washington Luiz, Km 235, BR-13563905 Sao Carlos, SP - Brazil

^[2] Univ Fed Sao Carlos, Dept Physiol Sci, Rodovia Washington, Luiz Km 235, BR-13563905 Sao Carlos, SP - Brazil

^[3] Fed Univ Tocantins, Campus Araguaina, Ave Paraguai S-N, BR-77824838 Araguaina, TO - Brazil

^[4] Univ Fed Sao Carlos, Chem Dept, Rodovia Washington Luiz, Km 235, BR-13565905 Sao Carlos, SP - Brazil

• Document type: Journal article
• Source: ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY; v. 208, JAN 15 2021.
• Web of Science Citations: 1

Abstract

The flavonoid metal-insecticide {[}Mg(hesp)2(phen)], denominated MgHP, has high potential for controlling agricultural pests. If applied in large scale, it may reach aquatic ecosystems and be harmful to the biota. This study evaluated the effects of MgHP in the gills of the Neotropical fish, Prochilodus lineatus by determining the activity of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione s-transferase (GST), and the levels of glutathione (GSH) and lipid peroxidation (LPO) after 24 and 96 h exposure to 0, 1, 10, 100 and 1000 mu g L-1. The histopathological changes with emphases to mitochondria-rich cells (MRC) were evaluated as well. After 24 h exposure the enzyme activities and the GSH and LPO levels were unchanged however, after 96 h exposure to high MgHP concentration (1000 mu g L-1), the GST activity and GSH levels increased. Oxidative stress measured as LPO levels did not occur after MgHP exposure in both periods. Gill tissue alterations increased after MgHP exposure to 10, 100 and 1000 mu g L-1. Cellular atrophy, pillar cells changes, filament epithelium hyperplasia and hypertrophy, lamellar epithelium hyperplasia were the most frequent histopathology. MRC in the filament epithelium decreased after exposure to 24 h and increased after 96 h indicating possible transitory osmo-ionic disruption. P. lineatus exhibited high tolerance to MgHP. The increased GST activity and GSH levels after 96 h exposure suggested possible MgHP accumulation and concentrationand time dependent response. Histopathology in the gills of exposed fish occurred at high MgHP concentrations. These results suggested that the MgHP into water, at high concentrations, affect the gills by changing GST activity, GSH levels and histology being useful biomarkers for MgHP water contamination. (AU)

• FAPESP's process: 12/25299-6 - Integrated studies for leaf cutting control
• Grantee: João Batista Fernandes
• Support Opportunities: Research Projects - Thematic Grants