3D-printed reduced graphene oxide/polylactic acid electrodes: A new prototyped platform for sensing and biosensing applications

Author(s)

Silva, Vinicius A. O. P. ^[1] ; Fernandes-Junior, Wilson S. ^[1] ; Rocha, Diego P. ^[2] ; Stefano, Jessica S. ^[2] ; Munoz, Rodrigo A. A. ^[2] ; Bonacin, Juliano A. ^[3] ; Janegitz, Bruno C. ^[1]

Affiliation

^[1] Univ Fed Sao Carlos, Dept Nat Sci Math & Educ, BR-13600970 Araras, SP - Brazil

^[2] Univ Fed Uberlandia, Inst Chem, BR-38400902 Uberlandia, MG - Brazil

^[3] Univ Estadual Campinas, Inst Chem, POB 6154, BR-13083970 Campinas, SP - Brazil

• Document type: Journal article
• Source: BIOSENSORS & BIOELECTRONICS; v. 170, DEC 15 2020.
• Web of Science Citations: 1

Abstract

This work presents a novel procedure involving the sequential chemical treatment to generate reduced graphene oxide (rGO) within 3D-printed polylactic acid (PLA) electrodes and their potential applications for sensing and biosensing. A new configuration of a compact all-3D-printed electrochemical device containing the three electrodes is presented, in which the working electrode was treated to generate rGO within PLA (rGO-PLA) after treatment within NaBH4. The rGO-PLA electrodes presented a notable current increase for the redox probe ferrocene-methanol in comparison with the same surface treated by dimethylformamide immersion. Also, the electrochemical impedance spectroscopic data that presented the lowest resistance to electron transfer for the proposed electrode. The electrochemical experiments were in accordance with Raman spectra and surface roughness obtained by atomic force microscopy images. As proofs-of-concept, the rGO-PLA electrode was applied for serotonin determination in synthetic urine using differential-pulse voltammetry with a limit of detection of 0.032 mu mol L-1. Also, the second application involved the fabrication of a tyrosinase-based biosensor capable of determining catechol in natural water samples with a limit of detection of 0.26 mu mol L-1. Based on both applications, the 3D-printed rGO-PLA showed to be an excellent platform for sensing and biosensing purposes.comment (AU)

• FAPESP's process: 17/23960-0 - Study of the mechanism of water oxidation by catalysts operating at pH7 and its incorporation in electrodes printed in 3D
• Grantee: Juliano Alves Bonacin
• Support Opportunities: Regular Research Grants
• FAPESP's process: 17/21097-3 - Bee-agriculture interactions: perspectives to sustainable use
• Grantee: Osmar Malaspina
• Support Opportunities: BIOTA-FAPESP Program - Thematic Grants
• FAPESP's process: 13/22127-2 - Development of novel materials strategic for integrated analytical devices
• Grantee: Lauro Tatsuo Kubota
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 19/26953-0 - Electrochemical sensors and biosensors printed in 3D using graphene filament for detection of phenolic compounds
• Grantee: Wilson da Silva Fernandes Junior
• Support Opportunities: Scholarships in Brazil - Scientific Initiation