Chemical composition, cytotoxicity and antimicrobial activity of selected plant-derived essential oils against fish pathogens

Author(s)

Assane, Inacio M. ^{[1, 2]} ; Valladao, Gustavo M. R. ^[3] ; Pilarski, Fabiana ^{[1, 4]}

Affiliation

^[1] Sao Paulo State Univ Unesp, Lab Microbiol & Parasitol Aquat Organisms, Aquaculture Ctr Unesp, BR-14884900 Jaboticabal, SP - Brazil

^[2] Univ Zambeze UniZambeze, Fac Ciencias Agr, Ulongue, Tete - Mozambique

^[3] Nilton Lins Univ, Manaus, Amazonas - Brazil

^[4] Sao Paulo State Univ UNESP, Lab Microbiol & Parasitol Aquat Organisms, Sch Agr & Vet Sci, Jaboticabal - Brazil

• Document type: Journal article
• Source: AQUACULTURE RESEARCH; v. 52, n. 2, p. 793-809, FEB 2021.
• Web of Science Citations: 0

Abstract

The use of essential oils (EOs) is a promising strategy for reducing antibiotic use in aquaculture. In this study, we investigated the chemical composition, in vitro cytotoxicity and antimicrobial activity of 16 commercial plant-derived EOs againstAeromonas hydrophila,A. jandaeiandCitrobacter freundiiisolated from diseased freshwater fish (Oreochromis niloticusandPterophyllum scalare). Efficacy of treatment with EOs and four antibiotics used in aquaculture: enrofloxacin, florfenicol, oxytetracycline and thiamphenicol, were compared. The chemical compositions of EOs were determined via high-resolution gas chromatography, and the in vitro cytotoxicity and antimicrobial activity were assessed using a haemolysis assay and the microdilution method respectively. Ninety-four components of EOs were identified. The principal components of most EOs were oxygenated monoterpene hydrocarbons. Most EOs possessed non-cytotoxic effects against fish erythrocytes at concentrations with strong (MIC <= 500 mu g/ml) and moderate (600 mu g/ml <= MIC <= 1,500 mu g/ml) antimicrobial activity.A. sativum,C. verum,O. basilicum,S. aromaticumandT. vulgarisEOs were the most active against microbial strains assessed. The present study highlights the potential of using EO to control bacterial disease in cultured fish. (AU)

• FAPESP's process: 19/22775-0 - Combination of thiamphenicol with florfenicol: pharmacokinetics and therapeutic efficacy in the treatment of Streptococcosis in Nile Tilapia (Oreochromis niloticus)
• Grantee: Inácio Mateus Assane
• Support Opportunities: Scholarships in Brazil - Doctorate