Combination of thiamphenicol with florfenicol: pharmacokinetics and therapeutic efficacy in the treatment of Streptococcosis in Nile Tilapia (Oreochromis niloticus)

• Grant number: 19/22775-0
• Support type: Scholarships in Brazil - Doctorate
• Effective date (Start): June 01, 2020
• Effective date (End): May 31, 2022
• Field of knowledge: Agronomical Sciences - Fishery Resources and Fishery Engineering - Aquaculture
• Principal researcher: Fabiana Pilarski
• Grantee: Inácio Mateus Assane
• Home Institution: Centro de Aquicultura (CAUNESP). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil

Abstract

Brazilian aquaculture production has increased significantly in the last two decades, mainly Tilapia Tilapia breeding. However, outbreaks of bacterial diseases have led to major economic losses; resulting in increased risks and uncertainties about the future of the activity and reduced investment. Given this situation, and the scarcity of effective therapeutic alternatives for the control of disease outbreaks, several antimicrobials are used illegally and inappropriately; resulting in therapeutic inefficacy of available antimicrobials, emergence and dispersion of antimicrobial resistance in fish farming. There are currently only two antibiotics approved for use in Aquaculture in Brazil, oxytetracycline and florfenicol (FFC), and only FFC is approved for the treatment of diseases in Tilapia. Given this scenario, the search for effective therapeutic alternatives for the treatment of bacterial diseases in fish farming is a necessity. Thus, we are going to study the pharmacokinetics (plasma) profile of thiamphenicol (TAP), FFC and florfenicol amine (FFCA) in Nile Tilapia after administration of the combination TAP-FFC; and the therapeutic efficacy of this combination in the treatment of Streptococcosis (caused by Streptococcus spp.) in Nile Tilapia. Streptococcosis is among the main diseases that affect Tilapia production (from fry (10g) to adult (1kg)). Satisfactory results on the therapeutic efficacy of the TAP: FFC combination in the treatment of various bacterial diseases in various species of livestock, including fish, suggest the potential of this combination as a therapeutic alternative for the treatment of bacterial diseases in Nile Tilapia. The results of this research are expected to contribute to the establishment of an effective Streptococcosis treatment protocol using the TAP: FFC combination in Tilapia. (AU)

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

VANECI-SILVA, DAIANE; ASSANE, INACIO MATEUS; ALVES, LINDOMAR DE OLIVEIRA; GOMES, FERNANDO CARDOSO; MORO, EVANDRO BILHA; KOTZENT, SUZANA; SILVA, ANDRE PITONDO; PILARSKI, FABIANA. Klebsiella pneumoniae causing mass mortality in juvenile Nile tilapia in Brazil: Isolation, characterization, pathogenicity and phylogenetic relationship with other environmental and pathogenic strains from livestock and human sources. Aquaculture, v. 546, JAN 15 2022. Web of Science Citations: 0.

ASSANE, I. M.; SANTOS-FILHO, N. A.; DE SOUSA, E. L.; DEARRUDA BRASIL, M. C. O.; CILLI, E. M.; PILARSKI, F. Cytotoxicity and antimicrobial activity of synthetic peptides alone or in combination with conventional antimicrobials against fish pathogenic bacteria. Journal of Applied Microbiology, v. 131, n. 4, p. 1762-1774, OCT 2021. Web of Science Citations: 1.

ASSANE, INACIO MATEUS; PRADA-MEJIA, KAREN DAYANA; GALLANI, SILVIA UMEDA; WEISER, NATASHA FERNANDES; RAMOS VALLADAO, GUSTAVO MORAES; PILARSKI, FABIANA. Enterogyrus spp. (Monogenea: Ancyrocephalinae) and Aeromonas jandaei co-infection associated with high mortality following transport stress in cultured Nile tilapia. TRANSBOUNDARY AND EMERGING DISEASES, SEP 2021. Web of Science Citations: 0.

DE SOUSA, ELIELMA LIMA; ASSANE, INACIO MATEUS; SANTOS-FILHO, NORIVAL ALVES; CILLI, EDUARDO MAFFUD; DE JESUS, RAPHAEL BARBETTA; PILARSKI, FABIANA. Haematological, biochemical and immunological biomarkers, antibacterial activity, and survival in Nile tilapia Oreochromis niloticus after treatment using antimicrobial peptide LL-37 against Streptococcus agalactiae. Aquaculture, v. 533, FEB 25 2021. Web of Science Citations: 0.

ASSANE, INACIO M.; VALLADAO, GUSTAVO M. R.; PILARSKI, FABIANA. Chemical composition, cytotoxicity and antimicrobial activity of selected plant-derived essential oils against fish pathogens. AQUACULTURE RESEARCH, v. 52, n. 2, p. 793-809, FEB 2021. Web of Science Citations: 0.