| Full text | |
| Author(s): |
Rueda Furlan, Joao Pedro
[1]
;
Lopes, Ralf
[1]
;
Lima Gonzalez, Irys Hany
[2]
;
Ramos, Patricia Locosque
[2]
;
von Zeska Kress, Marcia Regina
[1]
;
Stehling, Eliana Guedes
[1]
Total Authors: 6
|
| Affiliation: | [1] Univ Sao Paulo, Dept Anal Clin Toxicol & Bromatol, Fac Ciencias Farmaceut Ribeirao Preto, Sao Paulo - Brazil
[2] Fundacao Parque Zool Sao Paulo, Sao Paulo - Brazil
Total Affiliations: 2
|
| Document type: | Journal article |
| Source: | Veterinary Microbiology; v. 251, DEC 2020. |
| Web of Science Citations: | 0 |
| Abstract | |
The rapid dissemination of extended-spectrum beta-lactamases (ESBLs)-producing Enterobacterales from different spheres worldwide over recent years has become a serious problem in both human and veterinary medicine. CTXM-3-type ESBL has only been reported on few occasions, and in Brazil the bla(CTX-M-3) gene has been identified only once in clinical strains. In this study, we aimed to molecularly characterize a hypermucoviscous (hm), hyper virulent (hv), and extensively drug-resistant (XDR) Klebsiella pneumoniae strain isolated from a lung tissue culture of an infected elephant. The A246 strain belonged to ST2121 and presented hm phenotype, hypervirulenceassociated genes, and carried bla(CTX-M-3) and plasmid-mediated quinolone resistance genes (qnrB2 and qnrS1) on an IncFII-IncQ1-IncM1 multireplicon plasmid (pA246-CTX-M-3, similar to 162 kb). A novel genetic context of bla(CTXM-3), in which a 482-bp ISEcp1 was truncated by an IS26, was also harbored by pA246-CTX-M-3. Furthermore, in vivo experiments revealed that the hm/hv A246 strain killed 100 % of the Galleria mellonella larvae at 72 h post infection. Our findings evidence the intercontinental dissemination of a rare K. pneumoniae ST2121 and the multidrug resistance IncFII-IncQ1-IncM1 plasmid. Therefore, to the best of our knowledge, this is the first report of an XDR K. pneumoniae coproducing CTX-M-3, QnrB2, and QnrS1 isolated from captive wild animals. (AU) | |
| FAPESP's process: | 18/19539-0 - Molecular characterization of Escherichia coli isolates from the environment |
| Grantee: | Eliana Guedes Stehling |
| Support Opportunities: | Regular Research Grants |