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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Metabolomic study of marine Streptomyces sp.: Secondary metabolites and the production of potential anticancer compounds

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Author(s):
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Tangerina, Marcelo M. P. [1] ; Furtado, Luciana Costa [2] ; Leite, Vida M. B. [3] ; Bauermeister, Anelize [2, 4] ; Velasco-Alzate, Karen [2] ; Jimenez, Paula C. [5] ; Garrido, Leandro M. [3] ; Padilla, Gabriel [3] ; Lopes, Norberto P. [4] ; Costa-Lotufo, Leticia V. [2] ; Pena Ferreira, Marcelo J. [1]
Total Authors: 11
Affiliation:
[1] Univ Sao Paulo, Inst Biociencias, Dept Bot, Sao Paulo, SP - Brazil
[2] Univ Sao Paulo, Inst Ciencias Biomed, Dept Farmacol, Sao Paulo, SP - Brazil
[3] Univ Sao Paulo, Inst Ciencias Biomed, Dept Microbiol, Sao Paulo, SP - Brazil
[4] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Ciencias Biomol, NPPNS, Ribeirao Preto, SP - Brazil
[5] Univ Fed Sao Paulo, Inst Mar, Dept Ciencias Mar, Santos, SP - Brazil
Total Affiliations: 5
Document type: Journal article
Source: PLoS One; v. 15, n. 12 DEC 21 2020.
Web of Science Citations: 0
Abstract

Resorting to a One Strain Many Compounds (OSMAC) approach, the marine Streptomyces sp. BRB081 strain was grown in six different media settings over 1, 2, 3 or 7 days. Extractions of mycelium and broth were conducted separately for each media and cultivation period by sonication using methanol/acetone 1:1 and agitation with ethyl acetate, respectively. All methanol/acetone and ethyl acetate crude extracts were analysed by HPLC-MS/MS and data treatment was performed through GNPS platform using MZmine 2 software. In parallel, the genome was sequenced, assembled and mined to search for biosynthetic gene clusters (BGC) of secondary metabolites using the AntiSMASH 5.0 software. Spectral library search tool allowed the annotation of desferrioxamines, fatty acid amides, diketopiperazines, xanthurenic acid and, remarkably, the cyclic octapeptides surugamides. Molecular network analysis allowed the observation of the surugamides cluster, where surugamide A and the protonated molecule corresponding to the B-E isomers, as well as two potentially new analogues, were detected. Data treatment through MZmine 2 software allowed to distinguish that the largest amount of surugamides was obtained by cultivating BRB081 in SCB medium during 7 days and extraction of culture broth. Using the same data treatment, a chemical barcode was created for easy visualization and comparison of the metabolites produced overtime in all media. By genome mining of BRB081 four regions of biosynthetic gene clusters of secondary metabolites were detected supporting the metabolic data. Cytotoxic evaluation of all crude extracts using MTT assay revealed the highest bioactivity was also observed for extracts obtained in the optimal conditions as those for surugamides production, suggesting these to be the main active compounds herein. This method allowed the identification of compounds in the crude extracts and guided the selection of best conditions for production of bioactive compounds. (AU)

FAPESP's process: 17/16606-6 - Actinomycetes from Brazilian islands: a metabolomic approach in bioprospecting natural products with antitumor potential
Grantee:Marcelo Marucci Pereira Tangerina
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 15/17177-6 - Integrative approach on the sustainable prospection of marine natural products: from diversity to anticancer compounds
Grantee:Leticia Veras Costa Lotufo
Support Opportunities: BIOTA-FAPESP Program - Thematic Grants
FAPESP's process: 17/18235-5 - Anticancer potential of isolated substances from Streptomyces SP. recovered from ascidian Euherdmania SP
Grantee:Luciana Costa Furtado
Support Opportunities: Scholarships in Brazil - Master
FAPESP's process: 17/17648-4 - Integrative approach on the sustainable prospection of marine natural products: from diversity to anticancer compounds
Grantee:Anelize Bauermeister
Support Opportunities: Scholarships in Brazil - Post-Doctoral