ICAR Sugarcane Breeding Inst, Genet Transformat Lab, Div Crop Improvement, Coimbatore, Tamil Nadu - India
 Univ Sao Paulo, Ctr Nucl Energy Agr, Piracicaba, SP - Brazil
 Univ Fed Sao Paulo, Dept Biophys, Escola Paulista Med, Sao Paulo - Brazil
Total Affiliations: 8
International Journal of Biological Macromolecules;
JAN 15 2021.
Web of Science Citations:
Phytocystatins are tight-binding cysteine protease inhibitors produced by plants. The first phytocystatin described was isolated from Oryza sativa and, since then, cystatins from several plant species were reported, including from sugarcane. Sugarcane cystatins were unraveled in Sugarcane EST project database, after sequencing of cDNA libraries from various sugarcane tissues at different developmental stages and six sugarcane cystatins were cloned, expressed and characterized (CaneCPI-1 to CaneCPI-6). These recombinant proteins were produced in different expression systems and inhibited several cysteine proteases, including human cathepsins B and L, which can be involved in pathologies, such as cancer. In this review, we summarize a comprehensive history of all sugarcane cystatins, presenting an updated phylogenetic analysis; chromosomal localization, and genomic organization. We also present protein docking of CaneCPI-5 in the active site of human cathepsin B, insights about canecystatins structures; recombinant expression in different systems, comparison of their inhibitory activities against human cysteine cathepsins B, K, L, S, V, falcipains from Plasmodium falciparum and a cathepsin L-like from the sugarcane weevil Sphenophorus levis; and enlighten their potential and current applications in agriculture and health. (C) 2020 Elsevier B.V. All rights reserved. (AU)