Dexamethasone Does Not Inhibit Treadmill Training-Induced Angiogenesis in Myocardium: Role of MicroRNA-126 Pathway

Author(s)

Herrera, Naiara A. ^[1] ; Duchatsch, Francine ^[1] ; Tardelli, Lidieli P. ^[1] ; Dionisio, Thiago J. ^[2] ; Santos, Carlos F. ^[2] ; Amaral, Sandra L. ^{[3, 1]}

Affiliation

^[1] PIPGCF UFSCar UNESP, Joint Grad Program Physiol Sci, Sao Carlos, SP - Brazil

^[2] Univ Sao Paulo, Bauru Sch Dent, Dept Biol Sci, Bauru, SP - Brazil

^[3] Sao Paulo State Univ Unesp, Sch Sci, Dept Phys Educ, Bauru, SP - Brazil

• Document type: Journal article
• Source: Journal of Cardiovascular Pharmacology; v. 76, n. 6, p. 708-714, DEC 2020.
• Web of Science Citations: 1

Abstract

Dexamethasone (DEX) has important anti-inflammatory activities; however, it induces hypertension and skeletal muscle microcirculation rarefaction. Nevertheless, nothing is known about DEX outcomes on cardiac microcirculation. By contrast, exercise training prevents skeletal and cardiac microvessel loss because of microRNA expression and a better balance between their related angiogenic and apoptotic proteins in spontaneously hypertensive rats. The purpose of this study was to investigate whether DEX and/or exercise training could induce microRNA alterations leading to cardiac angiogenesis or microvascular rarefaction. Animals performed 8 weeks of exercise training and were treated with DEX (50 mu g/kg per day, subcutaneously) for 14 days. Cardiovascular parameters were measured, and the left ventricle muscle was collected for analyses. DEX treatment increased arterial pressure and did not cause cardiac microcirculation rarefaction. Treadmill training prevented the DEX-induced increase in arterial pressure. In addition, training, regardless of DEX treatment, increased microRNA-126 expression, phospho-protein kinase B/protein kinase B, and endothelial nitric oxide synthase levels associated with cardiac angiogenesis. In conclusion, this study suggests, for the first time, that treadmill training induces myocardial angiogenesis because of angiogenic pathway improvement associated with an increase in microRNA-126. Furthermore, DEX, per se, did not cause capillary density alterations and did not attenuate cardiac angiogenesis induced by training. (AU)

• FAPESP's process: 18/06998-7 - EXERCISE TRAINING PREVENTS RAREFACTION INDUCED BY DEXAMETHASONE: ROLE OF MITOCHONDRIA
• Grantee: Naiara Araújo Herrera
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• FAPESP's process: 17/00509-1 - Role of physical training on autonomic balance, arterial stiffness and rarefaction in spontaneously hypertensive rats treated with dexamethasone: role of miRNA
• Grantee: Sandra Lia do Amaral Cardoso
• Support Opportunities: Regular Research Grants
• FAPESP's process: 16/12532-5 - Exercise training effects on SHR treated with dexamethasone: role of miRNAs
• Grantee: Naiara Araújo Herrera
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 17/14405-3 - Influence of aerobic physical training on cardiac remodeling of spontaneously hypertensive rats treated with dexamethasone
• Grantee: Francine Duchatsch Ribeiro de Souza
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 15/03965-2 - Role of the renin-angiotensin system in different oral inflammatory models: an experimental interdisciplinary and clinical approach
• Grantee: Carlos Ferreira dos Santos
• Support Opportunities: Research Projects - Thematic Grants