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Exercise training effects on SHR treated with dexamethasone: role of miRNAs

Grant number: 16/12532-5
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): September 01, 2016
Effective date (End): August 31, 2020
Field of knowledge:Health Sciences - Physical Education
Principal researcher:Sandra Lia do Amaral Cardoso
Grantee:Naiara Araújo Herrera
Home Institution: Faculdade de Ciências (FC). Universidade Estadual Paulista (UNESP). Campus de Bauru. Bauru , SP, Brazil


Hypertension (H) is a multifactorial disease characterized by high and sustained levels of blood pressure (BP), classified as primary and secondary H. Dexamethasone (DEX) is a synthetic glucocorticoid with potent anti-allergic and anti-inflammatory effects and H is one of its unwanted side-effect. Spontaneously hypertensive rats (SHR) treated with DEX may have higher mortality risk due to H exacerbation. Microcirculation rarefaction is present in primary H (SHR) and in DEX induced H and is associated with angiogenic and apoptotic pathways alterations. More recently, miRNAs has being considered important in primary H genesis; however, its role in SHR treated with DEX has not yet being investigated. On the other hand, physical training (T) has been recommended crucial strategy to control hypertension. The aim of this study is to investigate if T is able to attenuate BP exacerbation in SHR treated with DEX and if this effect is associated with microcirculation miRNAs expression. Normotensive (Wistar) and hypertensive rats (SHR) will be submitted to eight weeks of T on a treadmill (60% of maximum physical capacity, 5 days a week, one hour per day) or kept sedentary. During 14 days, after eight weeks of T, animals will be treated with DEX (50µg/kg per day, s.c.) or saline. Body weight will be measured weekly during the T and daily during DEX treatment. Resting BP and arterial stiffness will be measured after DEX treatment. Subsequently, tibialis anterior muscle and left ventricle will be removed and processed for capillary density and capillary-to-fiber ratio, as well as, for VEGF, PI3K, eNOS, Bcl-2 and caspase-3-cleaved proteins production and miRNAs-16, -21, -126, -155, -205, -221 and -222 gene expression evaluations. Results will be presented as mean ± mean standard error. ANOVA two way and Tukey post-hoc will be performed for comparison among groups (p <0.05).

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
HERRERA, NAIARA A.; DUCHATSCH, FRANCINE; TARDELLI, LIDIELI P.; DIONISIO, THIAGO J.; SANTOS, CARLOS F.; AMARAL, SANDRA L. Dexamethasone Does Not Inhibit Treadmill Training-Induced Angiogenesis in Myocardium: Role of MicroRNA-126 Pathway. Journal of Cardiovascular Pharmacology, v. 76, n. 6, p. 708-714, DEC 2020. Web of Science Citations: 0.
HERRERA, NAIARA ARAUJO; DUCHATSCH, FRANCINE; KAHLKE, ALLISON; AMARAL, SANDRA LIA; VASQUEZ-VIVAR, JEANNETTE. In vivo vascular rarefaction and hypertension induced by dexamethasone are related to phosphatase PTP1B activation not endothelial metabolic changes. Free Radical Biology and Medicine, v. 152, p. 689-696, MAY 20 2020. Web of Science Citations: 0.
HERRERA, NAIARA A.; DUCHATSCH, FRANCINE; TARDELLI, LIDIELI P.; DIONISIO, THIAGO J.; SHINOHARA, ANDRE L.; SANTOS, CARLOS F.; AMARAL, SANDRA LIA. MicroRNA-126 upregulation, induced by training, plays a role in controlling microcirculation in dexamethasone treated rats. Molecular and Cellular Endocrinology, v. 505, APR 5 2020. Web of Science Citations: 0.

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