Biocompatible Lipid Polymer Cationic Nanoparticles for Antigen Presentation

Author(s)

Perez-Betancourt, Yunys ^[1] ; Tavora, Bianca de Carvalho Lins Fernandes ^[2] ; Faquim-Mauro, Eliana L. ^[2] ; Carmona-Ribeiro, Ana Maria ^[1]

Affiliation

^[1] Univ Sao Paulo, Inst Quim, Dept Bioquim, Biocolloids Lab, Ave Prof Lineu Prestes, 748 Butantan, BR-05508000 Sao Paulo - Brazil

^[2] Butantan Inst, Immunopathol Lab, Avr Vital Brasil, 1500, BR-05503900 Sao Paulo, SP - Brazil

• Document type: Journal article
• Source: POLYMERS; v. 13, n. 2 JAN 2021.
• Web of Science Citations: 0

Abstract

Biocompatible lipid polymer nanoparticles (NPs) previously used as antimicrobial agents are explored here as immuno-adjuvants. Poly (methyl methacrylate) (PMMA)/dioctadecyldimethylammonium bromide (DODAB)/poly (diallyldimethylammonium chloride) (PDDA) nanoparticles (NPs) were prepared by emulsion polymerization of methyl methacrylate (MMA) in the presence of DODAB and PDDA, with azobisisobutyronitrile (AIBN) as the initiator. NPs characterization after dialysis by dynamic light-scattering yielded 225 +/- 2 nm hydrodynamic diameter (Dz), 73 +/- 1 mV zeta-potential (zeta), and 0.10 +/- 0.01 polydispersity (P). Ovalbumin (OVA) adsorption reduced zeta to 45 +/- 2 mV. Balb/c mice immunized with NPs/OVA produced enhanced OVA-specific IgG1 and IgG2a, exhibited moderate delayed type hypersensitivity reaction, and enhanced cytokines production (IL-4, IL-10, IL-2, IFN-gamma) by cultured spleen cells. There was no cytotoxicity against cultured macrophages and fibroblasts. Advantages of the PMMA/DODAB/PDDA NPs were high biocompatibility, zeta-potential, colloidal stability, and antigen adsorption. Both humoral and cellular antigen-specific immune responses were obtained. (AU)

• FAPESP's process: 19/17685-2 - Supramolecular assemblies with immunoadjuvant or antimicrobial activity
• Grantee: Ana Maria Carmona-Ribeiro
• Support Opportunities: Regular Research Grants