The dynamics between in vitro culture and metabolism: embryonic adaptation to environmental changes

Author(s)

de Lima, Camila Bruna ^{[1, 2]} ; dos Santos, Erika Cristina ^[2] ; Ispada, Jessica ^{[1, 2]} ; Fontes, Patricia Kubo ^[3] ; Gouveia Nogueira, Marcelo Fabio ^{[3, 4]} ; Dias dos Santos, Charles Morphy ^[2] ; Milazzotto, Marcella Pecora ^{[1, 2]}

Affiliation

^[1] Univ Sao Paulo, Inst Biomed Sci, Sao Paulo - Brazil

^[2] Fed Univ ABC, Ctr Nat & Human Sci, Av Estados 5001, Bloco A, Lab 502-3, BR-09210580 Santo Andre, SP - Brazil

^[3] Sao Paulo State Univ, Inst Biosci, Dept Pharmacol, Lab PhytoPharmaTech, Campus Botucatu, Sao Paulo - Brazil

^[4] Sao Paulo State Univ, Sch Sci & Languages, Dept Biol Sci, Campus Assis, Sao Paulo - Brazil

• Document type: Journal article
• Source: SCIENTIFIC REPORTS; v. 10, n. 1 SEP 24 2020.
• Web of Science Citations: 0

Abstract

Previous studies have discussed the importance of an optimal range of metabolic activity during preimplantation development. To avoid factors than can trigger an undesirable trajectory, it is important to learn how nutrients and metabolites interact to help launching the correct developmental program of the embryo, and how much the in vitro culture system can impair this process. Here, using the bovine model, we describe a factorial experimental design used to investigate the biochemical and molecular signature of embryos in response to different combinations of morphological features-i.e. speed of development-and external stimuli during in vitro culture-i.e. different oxygen tensions and glucose supplementation. Our analyses demonstrate that the embryos present heterogeneous metabolic responses depending on early morphological phenotypes and the composition of their surroundings. However, despite the contribution of each single stimulus for the embryo phenotype, oxygen tension is determinant for such differences. The lower oxygen environment boosts the metabolism of embryos with faster kinetics, in particular those cultured in lower glucose concentrations. (AU)

• FAPESP's process: 12/50533-2 - GIFT: genomic improvement of fertilization traits in Danish and Brazilian cattle
• Grantee: Marcelo Fábio Gouveia Nogueira
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 17/18384-0 - METABOLOEPYGENETICS: THE STUDY OF THE BOVINE EMBRYO DEVELOPMENT UNDER A NEW PERSPECTIVE
• Grantee: Marcella Pecora Milazzotto
• Support Opportunities: Regular Research Grants
• FAPESP's process: 15/03381-0 - CELLULAR STRESS AND ITS RELATION WITH THE KINECTICS OF IN VITRO PRODUCED BOVINE EMBRYOS
• Grantee: Marcella Pecora Milazzotto
• Support Opportunities: Regular Research Grants
• FAPESP's process: 17/11768-8 - Phylogenetic systematics and biogeography of Diptera in the Atlantic Forest: analysis of form, time and space emphasizing the infraorder Tabanomorpha (Diptera, Brachycera)
• Grantee: Charles Morphy Dias dos Santos
• Support Opportunities: BIOTA-FAPESP Program - Regular Research Grants