| Full text | |
| Author(s): |
Delechiave, Giovanne
[1]
;
Naves, Alliny F.
[1]
;
Kolanthai, Elayaraja
[1]
;
da Silva, Rubens A.
[1]
;
Vlasman, Raphael C.
[1]
;
Petri, Denise F. S.
[1]
;
Torresi, Roberto M.
[1]
;
Catalani, Luiz H.
[1]
Total Authors: 8
|
| Affiliation: | [1] Univ Sao Paulo, Inst Quim, Dept Quim Fundamental, Av Prof Lineu Prestes 748, CP 26077, BR-05508000 Sao Paulo - Brazil
Total Affiliations: 1
|
| Document type: | Journal article |
| Source: | MATERIALS ADVANCES; v. 1, n. 6, p. 2043-2056, SEP 1 2020. |
| Web of Science Citations: | 0 |
| Abstract | |
In the past two decades, the fabrication of nanolayer coatings on polymeric materials has triggered considerable interest in the tissue-engineering field. Layer-by-Layer (LbL) assembly has attracted substantial attention due to being a convenient and low-cost method to coat materials with biomolecules while keeping their original biofunctionalities. This work aims to access a deeper understanding of the processes of adsorption and desorption of proteins by studying different architectures for the loading and release of model proteins on spin-coated flat surfaces of biodegradable polyesters such as poly(epsilon -caprolactone) (PCL) and poly(l-lactic acid) (PLLA) by the LbL technique. Surfaces of Si-wafers coated with PCL and PLLA were used as substrates for deposition of model proteins such as bovine serum albumin and lysozyme using heparin and chitosan as a pair of polyelectrolytes for LbL assembly. The analysis of the attributes of these protein reservoirs such as the protein layer position, layer thickness, surface morphology, protein adsorption and desorption quantification and kinetics was performed by ellipsometry, atomic force microscopy, fluorescence microscopy, and quartz-crystal microbalance with enhanced dissipation measurements. A homogenous LbL coating on the surface of the polyester films was achieved by assembling ten layer-pairs of reservoirs. The release profiles were evaluated, and the results showed an intricate dependence on the protein volume charge density and its relation to coacervation/complexation processes with both polyelectrolytes. Our results clearly suggest that the protein delivery kinetics can be fully controlled by precisely positioning the protein within the assembly (bottom vs. top position). They also suggest that the size and total charge of the protein are key factors for controlling its total load in the assembly. (AU) | |
| FAPESP's process: | 18/14532-8 - Development of transfection device from nanoparticles functionalized by LbL for specific delivery of CRISPR/Cas9 system |
| Grantee: | Giovanne Delechiave |
| Support type: | Scholarships in Brazil - Doctorate (Direct) |
| FAPESP's process: | 11/21442-6 - Synthetic and natural polymers applied to tissue engineering |
| Grantee: | Luiz Henrique Catalani |
| Support type: | Research Projects - Thematic Grants |
| FAPESP's process: | 17/06394-1 - Muti-user equipment approved in grant 2014/22136-4: isothermal titration calorimeter (ITC) for the determination of thermodynamic parameters of molecular interactions |
| Grantee: | Omar Abou El Seoud |
| Support type: | Multi-user Equipment Program |
| FAPESP's process: | 15/19694-8 - Bioactivity of multi-layer membranes loaded with various protein markers for tissue engineering applications |
| Grantee: | Elayaraja Kolanthai |
| Support type: | Scholarships in Brazil - Post-Doctorate |
| FAPESP's process: | 18/13492-2 - Synthetic and natural scaffolds applied to regenerative medicine |
| Grantee: | Luiz Henrique Catalani |
| Support type: | Research Projects - Thematic Grants |
| FAPESP's process: | 15/10009-0 - Protein reservoirs from multi layers assembled onto PLLA and PCL films |
| Grantee: | Giovanne Delechiave |
| Support type: | Scholarships in Brazil - Scientific Initiation |