Monocytes from preeclamptic women previously treated with silibinin attenuate oxidative stress in human endothelial cells

Author(s)

Gomes, Virginia Juliani ^[1] ; Nunes, Priscila Rezeck ^[1] ; Haworth, Sarah McCann ^[2] ; Sandrim, Valeria Cristina ^[3] ; Peracoli, Jose Carlos ^[1] ; Peracoli, Maria Terezinha S. ^[3] ; Carlstrom, Mattias ^[2]

Affiliation

^[1] Sao Paulo State Univ Unesp, Botucatu Med Sch, Dept Gynecol & Obstet, BR-18618687 Botucatu, SP - Brazil

^[2] Karolinska Inst, Dept Physiol & Pharmacol, Stockholm - Sweden

^[3] Sao Paulo State Univ Unesp, Inst Biosci Botucatu, Dept Biol & Chem Sci, Botucatu, SP - Brazil

• Document type: Journal article
• Source: HYPERTENSION IN PREGNANCY; v. 40, n. 2 FEB 2021.
• Web of Science Citations: 0

Abstract

Objective: To investigate whether the supernatant from monocytes of preeclamptic and normotensive pregnant women, cultured in vitro with silibinin, can modulate oxidative stress in HUVEC. Methods: Concentrations of IL-1 beta, IL-10, and TNF-alpha in monocyte culture supernatants were determined by ELISA. HUVEC and their supernatant cultures were employed for determination of NO, nitrite and nitrate, lipid peroxidation, and hemeoxygenase-1 (HO-1). Results: HUVEC treatment with supernatant of preeclamptic monocytes cultured with silibinin produced increased levels of nitrite, reduced lipid peroxidation, and increased HO-1. Conclusion: Supernatant of monocytes from preeclamptic women induce oxidative stress in HUVEC which can be reduced by silibinin treatment. (AU)

• FAPESP's process: 19/10250-0 - Effects of supernatant from monocytes of pre-eclamptic women treated with sibilin on oxidative stress and nitric oxide availability in endothelial cells
• Grantee: Virgínia Juliani Gomes
• Support Opportunities: Scholarships abroad - Research Internship - Master's degree