A spot test for direct quantification of acid green 16 adsorbed on a molecularly imprinted polymer through diffuse reflectance measurements

Author(s)

Mortari, Bianca ^[1] ; Khan, Sabir ^{[1, 2, 3]} ; Wong, Ademar ^[1] ; Sotomayor, Maria D. P. T. ^{[1, 2]}

Affiliation

^[1] Sao Paulo State Univ UNESP, Inst Chem, Araraquara, SP - Brazil

^[2] Natl Inst Alternat Technol Detect Toxicol Evaluat, Araraquara, SP - Brazil

^[3] Natl Univ Engn, Fac Sci, Lab Phys Chem Res, Av Tupac Amaru 210, Lima - Peru

• Document type: Journal article
• Source: ANALYTICAL METHODS; v. 13, n. 4, p. 453-461, JAN 28 2021.
• Web of Science Citations: 0

Abstract

This work describes a novel technique for the direct quantification of Acid Green 16 (AG16) adsorbed on a molecularly imprinted polymer (MIP) through the application of diffuse reflectance spectrophotometry (DRS) directly in a solid material. The MIP was synthesized by a bulk method using 1-vinylimidazole as the functional monomer. To conduct DRS analysis, adsorption assays were performed through the application of the MIP in a solution containing different concentrations of AG16 for 120 minutes; subsequently, the MIP was left to dry and a certain quantity of the polymer was analyzed. Under optimized conditions, a linear concentration range of 1.0 mu mol L-1 to 10.0 mu mol L-1 and limits of detection and quantification of 0.3 mu mol L-1 and 1.0 mu mol L-1, respectively, were obtained. The repeatability and reproducibility of the method were evaluated and RSD values lower than 4% were obtained. Selectivity studies allowed finding imprinting factor values of 1.9, 2.6, 1.1, and 1.1 for AG16, Direct yellow 50, Acid Blue 1, and Brilliant Green, respectively. The proposed method was applied toward the analysis of river water and textile industry effluents. The advantage and novelty of the technique lie in the fact that the amount of the analyte adsorbed on the selective polymer is directly measured on the solid material for AG16 and not indirectly via the remaining solution as it has always been carried out in previous studies reported in the literature. The findings show that the proposed technique is relatively simple, novel and highly versatile for the quantification of analytes adsorbed on MIPs, as well as for the analysis of the material of interest and quantification of diverse analytes in different matrices. (AU)

• FAPESP's process: 19/00677-7 - RADes-MIP - preparation and synthesis of materials for development of rapid analysis devices for monitoring emerging pollutants using highly selective biomimetic polymers based on molecular printing technology
• Grantee: Maria Del Pilar Taboada Sotomayor
• Support Opportunities: Regular Research Grants
• FAPESP's process: 14/50945-4 - INCT 2014: National Institute for Alternative Technologies of Detection, Toxicological Evaluation and Removal of Micropollutants and Radioactivies
• Grantee: Maria Valnice Boldrin
• Support Opportunities: Research Projects - Thematic Grants