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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Comparative analysis of two recombinant LPMOs from Aspergillus fumigatus and their effects on sugarcane bagasse saccharification

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Velasco, Josman [1] ; Pellegrini, Vanessa de Oliveira Arnoldi [2] ; Sepulchro, Ana Gabriela Veiga [2] ; Kadowaki, Marco Antonio Seiki [3] ; Santo, Melissa Cristina Espirito [2] ; Polikarpov, Igor [2] ; Segato, Fernando [1]
Total Authors: 7
[1] Univ Sao Paulo, Lorena Sch Engn, Dept Biotechnol, Estr Municipal Campinho S-N, BR-12602810 Lorena, SP - Brazil
[2] Univ Sao Paulo, Sao Carlos Inst Phys, Sao Carlos, SP - Brazil
[3] Univ Libre Bruxelles, EIB, Brussels - Belgium
Total Affiliations: 3
Document type: Journal article
Source: Enzyme and Microbial Technology; v. 144, MAR 2021.
Web of Science Citations: 1

Lytic polysaccharide monooxygenases (LPMOs) have been introduced into industrial cocktails used for biomass saccharification due to their capacity to boost enzymatic conversion of recalcitrant cellulose. The genome of the thermotolerant ascomycete Aspergillus fumigatus encodes 7 genes for LPMOs that belong to auxiliary activity family 9 (AA9). Here, we cloned, successfully expressed and performed biochemical evaluation of two CBM-less A. fumigatus LPMOs (AfAA9A and AfAA9B). A high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) analysis demonstrated that AfAA9A and AfAA9B are able to oxide cellulose at C1 and C1/C4 positions, respectively. Synergic effects of LPMOs (separately and in combination) with cellulases were investigated. Supplementation of Celluclast 1.5 L with a low concentration of AfAA9B improved in 20 % the saccharification of sugarcane bagasse pretreated by steam explosion (SEB), while AfAA9A did not improvethe saccharification. Analysis of the hydrolyzed biomass by confocal laser scanning microscopy (CLSM) showed the LPMOs are promoting lignin oxidation in the lignocellulosic material. This study complements the available results concerning the utilization of LPMOs in the enzymatic saccharification of lignocellulosic biomass. (AU)

FAPESP's process: 14/06923-6 - Sugar cane biomass recalcitrance: basic knowledge related to the cell wall construction, pretreatment and enzymatic digestion, applied for the development of innovative biorefinery models
Grantee:Andre Luis Ferraz
Support type: Program for Research on Bioenergy (BIOEN) - Thematic Grants
FAPESP's process: 14/18714-2 - Enzymatic oxidation of sugarcane bagasse: discovery, characterization and new application of oxidative enzymes active in carbohydrates, applied to the enhancement of a fungal cell factory
Grantee:Fernando Segato
Support type: Program for Research on Bioenergy (BIOEN) - Young Investigators Grants
FAPESP's process: 19/01165-0 - New recombinant PMOS from Aspergillus fumigatus: biochemical characterization and its effect on saccharification of sugarcane bagasse with different pre-treatments
Grantee:Josman Andrey Velasco Mendoza
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 19/22284-7 - Biochemical, functional characterization and application of polysaccharide lytic monoxygenases identified in transcriptome and secretoma studies of filamentous fungi cultivated in sugarcane bagasse
Grantee:Fernando Segato
Support type: Program for Research on Bioenergy (BIOEN) - Regular Program Grants
FAPESP's process: 17/50025-0 - Development of lignin active enzyme mixture to produce sugars from lignocellulosic biomass for production of fuels, chemicals and bio based materials
Grantee:Fernando Segato
Support type: Regular Research Grants
FAPESP's process: 15/13684-0 - Structural and functional studies of enzymes that participate in complex carbohydrates synthesis and degradation
Grantee:Igor Polikarpov
Support type: Research Projects - Thematic Grants