Topical formulations containing Copaifera duckei Dwyer oleoresin improve cutaneous wound healing

Author(s):Show less -

Goncalves Dias, Fernanda Gosuen ^[1] ; Pereira, Lucas de Freitas ^[1] ; Furtado, Ricardo Andrade ^[1] ; Magalhaes, Georgia Mode ^[2] ; Miguel, Marina Pacheco ^[3] ; Gosuen Goncalves Dias, Luis Gustavo ^[4] ; Jorge, Adriana Torrecilhas ^[1] ; Honsho, Cristiane dos Santos ^[1] ; Ambrosio, Sergio Ricardo ^[5] ; Bastos, Jairo Kenupp ^[6] ; Carrijo, Micaela Silva ^[1] ; Tavares, Denise Crispim ^[5]

Affiliation

^[1] Univ Franca, Dept Vet Med, Franca, SP - Brazil

^[2] Inst Fed Minas Gerais, Dept Vet Med, Muzambinho, MG - Brazil

^[3] Univ Fed Goias, Dept Vet Med, Goiania, Go - Brazil

^[4] Univ Estadual Paulista, Dept Vet Med, Jaboticabal, SP - Brazil

^[5] Univ Franca, Dept Sci, Franca, SP - Brazil

^[6] Univ Sao Paulo, Dept Pharm, Ribeirao Preto, SP - Brazil

• Document type: Journal article
• Source: AVICENNA JOURNAL OF PHYTOMEDICINE; v. 11, n. 2, p. 120-133, MAR-APR 2021.
• Web of Science Citations: 0

Abstract

Objective: Evaluation of the healing and toxicological effects of Copaifera duckei Dwyer oleoresin (CDO). Materials and Methods: Rodents with skin lesions were divided into nine groups, including daily treatments with 1, 3 and 10% CDO, collagenase, antibiotic ointment and control groups, for 14 days. Results: Treatment with 10% CDO reduced skin edema and hyperplasia, demonstrating anti-inflammatory effect of the oil. Reduction in the wound area was observed, indicating the healing effect of CDO. Histopathological analysis showed increases in angiogenesis and re-epithelialization in animals treated with the highest concentration. On the other hand, no alterations in ulcerations, inflammatory infiltrate, hemorrhage, congestion, degeneration, percentage of collagen fibers, number of cells stained with anti-macrophage migration inhibitory factor, or density of area stained with anti-collagen I and III were found. Toxicogenetic analysis revealed no differences in micronucleus frequencies or in the ratio of polychromatic eiythrocytes to total eiythrocytes between treated and negative control, demonstrating the absence of genotoxicity and cytotoxicity, respectively. There was no difference in levels of liver enzymes among groups, indicating the absence of hepatotoxicity. Conclusion: Formulations of CDO exerted beneficial effects on the stages of cutaneous wound healing and are promising options for the treatment of wounds. (AU)

• FAPESP's process: 11/13630-7 - Chemical and pharmacological validation of extracts and active compounds of Copaifera species
• Grantee: Jairo Kenupp Bastos
• Support Opportunities: Research Projects - Thematic Grants