Haplotypes traceability and genetic variability of the breeding population of pacu (Piaractus mesopotamicus) revealed by mitochondrial DNA

Author(s)

Milena V. de Freitas ^[1] ; Raquel B. Ariede ^[2] ; Milene E. Hata ^[3] ; Vito A. Mastrochirico-Filho ^[4] ; Felipe Del Pazo ^[5] ; Gabriela V. Villanova ^[6] ; Fernando F. Mendonça ^[7] ; Fábio Porto-Foresti ^[8] ; Diogo T. Hashimoto ^[9]

Affiliation

^[1] Universidade Estadual Paulista "Júlio de Mesquita Filho". Centro de Aquicultura - Brasil

^[2] Universidade Estadual Paulista "Júlio de Mesquita Filho". Centro de Aquicultura - Brasil

^[3] Universidade Estadual Paulista "Júlio de Mesquita Filho". Centro de Aquicultura - Brasil

^[4] Universidade Estadual Paulista "Júlio de Mesquita Filho". Centro de Aquicultura - Brasil

^[5] Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro Científico y Tecnológico Acuario del Río Paraná - Argentina

^[6] Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro Científico y Tecnológico Acuario del Río Paraná - Argentina

^[7] Universidade Federal de São Paulo. Instituto do Mar - Brasil

^[8] Universidade Estadual Paulista "Júlio de Mesquita Filho". Faculdade de Ciências - Brasil

^[9] Universidade Estadual Paulista "Júlio de Mesquita Filho". Centro de Aquicultura - Brasil

• Document type: Journal article
• Source: GENETICS AND MOLECULAR BIOLOGY; v. 44, n. 1 2021-03-12.

Abstract

Abstract The main objective of this study was to estimate the genetic diversity levels and haplotype traceability in pacu Piaractus mesopotamicus from the breeding program located in Brazil by analyses of the mitochondrial DNA control region (mtDNA). Moreover, broodstocks from eight commercial fish farms were used for comparative evaluation, four from Brazil (Br1-Br4) and four from Argentina (Ar1-Ar4). The descriptive results revealed 47 polymorphic sites and 51 mutations, which evidenced 34 haplotypes. Ten haplotypes were shared among fish farms and 24 were exclusive. The nucleotide diversity (π) ranged from 0.00031 to 0.01462 and haplotype diversity (Hd) from 0.125 to 0.868. The analysis of molecular variance (AMOVA) indicated high structure present in the analyzed stocks (F ST = 0.13356 and ФST = 0.52707). The genetic diversity was high in most of the commercial broodstocks, especially those from Brazil. We observed seven haplotypes in the genetic breeding population, of which four were exclusive and three shared among the commercial fish farms. The genetic diversity was moderate (π = 0.00265 and Hd = 0.424) and considered appropriated for this breeding population of pacu. Our results provide support for the genetic diversity maintenance and mtDNA traceability of pacu commercial broodstocks. (AU)

• FAPESP's process: 15/14185-8 - Analysis of the mitochondrial DNA as a resource for the pre-breeding program of the pacu Piaractus mesopotamicus
• Grantee: Milena Vieira de Freitas
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 14/03772-7 - Transcriptome characterization and SNPs discovery in species of Serrasalmidae: genetic subsidies for aquaculture
• Grantee: Diogo Teruo Hashimoto
• Support Opportunities: Regular Research Grants