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Analysis of the mitochondrial DNA as a resource for the pre-breeding program of the pacu Piaractus mesopotamicus

Grant number: 15/14185-8
Support Opportunities:Scholarships in Brazil - Master
Effective date (Start): October 01, 2015
Effective date (End): July 31, 2017
Field of knowledge:Agronomical Sciences - Fishery Resources and Fishery Engineering - Aquaculture
Principal Investigator:Diogo Teruo Hashimoto
Grantee:Milena Vieira de Freitas
Host Institution: Centro de Aquicultura (CAUNESP). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil


The Brazilian aquaculture has experienced significant growth in recent years. However, the national potential of production will certainly be better explored with the use of products resulting from breeding programs. The pacu, Piaractus mesopotamicus (Characiformes, Serrasalmidae), is a fish of occurrence in the Paraná-Paraguay River Basin and it is one of the main native species used for production in aquaculture. The use of molecular markers in fish farming has been shown to be essential, especially for genetic monitoring of populations and pre-breeding programs. The mitochondrial molecular markers (mtDNA) have a higher rate of mutations and exclusively maternal inheritance, being useful for monitoring the genetic variability of a population and generate information about the founder stock. The objective of this study is to analyze the genetic variability of cultivated stock of Piaractus mesopotamicus using mitochondrial molecular markers (D-loop region), in order to know the genetic profile of farmed animals. Then, we intended to generate subsidies to drive crossings in order to increase the population variability and improvements in genetic management in production systems. The expected result is the generation of haplotypes that can be used in the traceability of individuals resulting from breeding programs. In addition, the results of the present project may detect potential ex-situ genetic banks, formulating biological conservation strategies.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
MILENA V. DE FREITAS; RAQUEL B. ARIEDE; MILENE E. HATA; VITO A. MASTROCHIRICO-FILHO; FELIPE DEL PAZO; GABRIELA V. VILLANOVA; FERNANDO F. MENDONÇA; FÁBIO PORTO-FORESTI; DIOGO T. HASHIMOTO. Haplotypes traceability and genetic variability of the breeding population of pacu (Piaractus mesopotamicus) revealed by mitochondrial DNA. GENETICS AND MOLECULAR BIOLOGY, v. 44, n. 1, . (15/14185-8, 14/03772-7)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
FREITAS, Milena Vieira de. Analysis of the mitochondrial DNA as a resource for the pre-breeding program of the pacu Piaractus mesopotamicus. 2017. Master's Dissertation - Universidade Estadual Paulista (Unesp). Instituto de Biociências. Botucatu Botucatu.

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