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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Dynamic sex chromosome expression in Drosophila male germ cells

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Author(s):
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Mahadevaraju, Sharvani [1] ; Fear, Justin M. [1] ; Akeju, Miriam [2] ; Galletta, Brian J. [3] ; Pinheiro, Mara M. L. S. [4] ; Avelino, Camila C. [4] ; Cabral-de-Mello, Diogo C. [5] ; Conlon, Katie [2] ; Dell'Orso, Stafania [6] ; Demere, Zelalem [2] ; Mansuria, Kush [2] ; Mendonca, Carolina A. [4] ; Palacios-Gimenez, Octavio M. [4, 7, 8] ; Ross, Eli [2] ; Savery, Max [1] ; Yu, Kevin [2] ; Smith, Harold E. [9] ; Sartorelli, Vittorio [6] ; Yang, Haiwang [1, 10] ; Rusan, Nasser M. [3] ; Vibranovski, Maria D. [4] ; Matunis, Erika [2] ; Oliver, Brian [1]
Total Authors: 23
Affiliation:
[1] NIDDK, Lab Cellular & Dev Biol, NIH, Bethesda, MD 20892 - USA
[2] Johns Hopkins Univ, Sch Med, Dept Cell Biol, 725 N Wolfe St, Baltimore, MD 21205 - USA
[3] NHLBI, Cell Biol & Physiol Ctr, NIH, Bldg 10, Bethesda, MD 20892 - USA
[4] Univ Sao Paulo, Dept Genet & Evolutionary Biol, Inst Biosci, BR-05508090 Sao Paulo, SP - Brazil
[5] UNESP Univ Estadual Paulista, Dept Biol Geral & Aplicada, Inst Biociencias IB, BR-13506900 Rio Claro, SP - Brazil
[6] NIAMSD, Lab Muscle Stem Cells & Gene Regulat, NIH, Bethesda, MD 20892 - USA
[7] Uppsala Univ, Dept Evolutionary Biol, Evolutionary Biol Ctr, S-75236 Uppsala - Sweden
[8] Uppsala Univ, Dept Organismal Biol, Evolutionary Biol Ctr, Systemat Biol, S-75236 Uppsala - Sweden
[9] NIDDK, Genom Core, NIH, Bethesda, MD 20892 - USA
[10] Northwestern Univ, Dept Pharmacol, Feinberg Sch Med, Chicago, IL 60611 - USA
Total Affiliations: 10
Document type: Journal article
Source: NATURE COMMUNICATIONS; v. 12, n. 1 FEB 9 2021.
Web of Science Citations: 0
Abstract

Given their copy number differences and unique modes of inheritance, the evolved gene content and expression of sex chromosomes is unusual. In many organisms the X and Y chromosomes are inactivated in spermatocytes, possibly as a defense mechanism against insertions into unpaired chromatin. In addition to current sex chromosomes, Drosophila has a small gene-poor X-chromosome relic (4(th)) that re-acquired autosomal status. Here we use single cell RNA-Seq on fly larvae to demonstrate that the single X and pair of 4(th) chromosomes are specifically inactivated in primary spermatocytes, based on measuring all genes or a set of broadly expressed genes in testis we identified. In contrast, genes on the single Y chromosome become maximally active in primary spermatocytes. Reduced X transcript levels are due to failed activation of RNA-Polymerase-II by phosphorylation of Serine 2 and 5. Sex chromosome gene content and expression is unusual. Here the authors use single cell RNA-Seq on Drosophila larvae to demonstrate that the single X and pair of 4th chromosomes are specifically inactivated in primary spermatocytes, while genes on the single Y chromosome become maximally active in primary spermatocytes. (AU)

FAPESP's process: 15/20844-4 - The role of gametogenesis on the origin and evolution of new genes
Grantee:Maria Dulcetti Vibranovski
Support Opportunities: Research Grants - Young Investigators Grants
FAPESP's process: 17/14923-4 - A study on the functions of new Y-linked genes through expression analysis of Drosophila's spermatogenesis
Grantee:Carolina de Athayde Mendonça
Support Opportunities: Scholarships in Brazil - Master
FAPESP's process: 17/26609-2 - Study of meiotic X chromosome inactivation by gene expression analyses of the Drosophila melanogaster spermatogenesis
Grantee:Camila Correia Avelino
Support Opportunities: Scholarships in Brazil - Master
FAPESP's process: 15/16661-1 - Sex and B chromosome enigmas: model systems for the study of chromosome and genome evolution
Grantee:César Martins
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 13/08028-1 - CEGH-CEL - Human Genome and Stem Cell Research Center
Grantee:Mayana Zatz
Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC
FAPESP's process: 19/15212-0 - Study of meiotic X chromosome inactivation in Drosophila spp. spermatogenesis
Grantee:Camila Correia Avelino
Support Opportunities: Scholarships in Brazil - Doctorate (Direct)