Chemometrics unraveling nutrient dynamics during soybean seed germination

Author(s)

Romeu, Sara Luiza Zachi ^[1] ; Marques, Joao Paulo Rodrigues ^[1] ; Montanha, Gabriel Sgarbiero ^[1] ; de Carvalho, Hudson Wallace Pereira ^[1] ; Pereira, Fabiola Manhas Verbi ^{[2, 3]}

Affiliation

^[1] Univ Sao Paulo, Lab Nucl Instrumentat LIN, Ctr Nucl Energy Agr CENA, BR-13416000 Piracicaba, SP - Brazil

^[2] Natl Inst Alternat Technol Detect Toxicol Assessm, BR-14800060 Araraquara, SP - Brazil

^[3] Sao Paulo State Univ UNESP, Grp Alternat Analyt Approaches GAAA, Bioenergy Res Inst IPBEN, Inst Chem, BR-14800060 Araraquara, SP - Brazil

• Document type: Journal article
• Source: Microchemical Journal; v. 164, MAY 2021.
• Web of Science Citations: 0

Abstract

Laboratory microprobe X-ray fluorescence (?-XRF) spectrometry is a powerful analytical technique able to detect trace elements in biological samples. The present study compared univariate and multivariate strategies to investigate the elements detected with ?-XRF during soybean (Glycine max (L.) Merrill) germination. The conventional approach, i.e., univariate, showed that the studied micronutrients (Mn, Fe, and Zn) were located mainly on the radicle tip, while the macronutrients (P, S, K, and Ca) were spread through the seed. The score maps, as well the correlation maps from a covariance matrix, were able to evaluate variations of chemical elements, such as P, S, K, Ca, Fe, Mn, Zn, and Rh-scattering effects through the embryo, leading the first glance at elemental distribution and remobilization during soybean seed germination. (AU)

• FAPESP's process: 15/19121-8 - Multi-User Equipment approved in grant 2015/05942-0: micro X-Ray Fluorescence system
• Grantee: Hudson Wallace Pereira de Carvalho
• Support Opportunities: Multi-user Equipment Program
• FAPESP's process: 14/50945-4 - INCT 2014: National Institute for Alternative Technologies of Detection, Toxicological Evaluation and Removal of Micropollutants and Radioactivies
• Grantee: Maria Valnice Boldrin
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 20/07721-9 - Time-resolved element mapping and characterization of storage proteins during soybean seeds development: a bottom-up strategy to increase protein content and quality
• Grantee: Gabriel Sgarbiero Montanha
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• FAPESP's process: 19/01102-8 - Multi-user equipament approved in grant 2018/18212-8: X-Ray Fluorescence spectrometer
• Grantee: Fabiola Manhas Verbi Pereira
• Support Opportunities: Multi-user Equipment Program