The protective effect of Artepillin C against lipid oxidation on model membranes

Author(s)

Pazin, Wallance Moreira ^[1] ; Marques Ruiz, Gilia Cristine ^[1] ; dos Santos, Marcelo Jose ^[1] ; Benites Aoki, Pedro Henrique ^[2] ; Ito, Amando Siuiti ^[3] ; Leopoldo Constantino, Carlos Jose ^[1]

Affiliation

^[1] Sao Paulo State Univ UNESP, Sch Technol & Appl Sci, BR-19060900 Presidente Prudente, SP - Brazil

^[2] Sao Paulo State Univ UNESP, Sch Sci Humanities & Languages, BR-19806900 Assis, SP - Brazil

^[3] Univ Sao Paulo, Fac Philosophy Sci & Letters Ribeirao Preto, BR-14040901 Ribeirao Preto, SP - Brazil

• Document type: Journal article
• Source: JOURNAL OF MOLECULAR LIQUIDS; v. 324, FEB 15 2021.
• Web of Science Citations: 0

Abstract

Brazilian green propolis is awell-known therapeutic product, commonly used in folk medicine. Artepillin C is the major compound of Brazilian green propolis and has received considerable attention owing to its lipophilic affinity and antioxidant activity, enabling the use against lipid oxidation caused by free radicals, which is a first step before degenerative diseases. The protective effect of Artepillin C against lipid oxidation was evaluated here on models of lipid membranes based on Langmuir monolayers and giant unilamellar vesicles (GUVs) formed of 1,2-dioleoyl-sn-glycero-3-phosphocholine DOPC under oxidative stress induced by the photoactivated erythrosin. Our findings show that the lipophilic character of Artepillin C allows the donation of a hydrogen atom of the phenolic hydroxyl group to the lipid radical of both mono and bilayer, avoiding the formation of truncated aldehyde lipids, interrupting oxidative reactions mediated by reactive oxygen species (ROS), therefore playing a role as an antioxidant compound in the lipid environment. The affinity of Artepillin C for lipid structures, together with its antioxidant potential, preclude the lipid peroxidation caused by reactive species. (C) 2020 Elsevier B.V. All rights reserved. (AU)

• FAPESP's process: 16/09633-4 - Mechanism of action of the bioactive compound Artepillin C in membrane biomimetic systems and cytoplasmic membrane of tumor cells
• Grantee: Wallance Moreira Pazin
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 18/22214-6 - Towards a convergence of technologies: from sensing and biosensing to information visualization and machine learning for data analysis in clinical diagnosis
• Grantee: Osvaldo Novais de Oliveira Junior
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 18/16713-0 - Photodynamic therapy: from molecular effects in model systems toward photodynamic efficiency in cell culture
• Grantee: Pedro Henrique Benites Aoki
• Support Opportunities: Regular Research Grants