New sensitive methods for fraud detection in buffalo dairy products

Author(s)

Azevedo, Bianca Taina ^[1] ; Vercesi Filho, Anibal Eugenio ^[1] ; Gutmanis, Gunta ^[1] ; Verissimo, Cecilia Jose ^[1] ; Katiki, Luciana Morita ^[1] ; Okino, Cintia Hiromi ^[2] ; de Sena Oliveira, Marcia Cristina ^[2] ; Giglioti, Rodrigo ^[1]

Affiliation

^[1] Inst Zootecnia IZ, Rua Heitor Penteado 56, BR-13380011 Nova Odessa, SP - Brazil

^[2] EMBRAPA Pecuaria Sudeste, Rodovia Washington Luiz, Km 234, BR-13560970 Sao Carlos, SP - Brazil

• Document type: Journal article
• Source: INTERNATIONAL DAIRY JOURNAL; v. 117, JUN 2021.
• Web of Science Citations: 1

Abstract

The rising consumption of buffalo milk derivatives in recent years has highlighted the search for buffaloes in Brazilian livestock farming. These products have greater added value compared with common dairy milk products. Consequently, addition of variable amounts of cows' milk during manufacture of buffalo dairy products may occur, which constitutes fraud by product adulteration. Thus, the present study developed and standardised a DNA extraction protocol for application on different dairy derivatives and two methods based on real-time PCR for fraud identification: high-resolution melting (HRM) and rhAmp (R) SNP system. The extraction method allowed to extraction of DNA from eleven different dairy products. The sensitivity of the rhAmp method (1%) was five times higher than HRM assay (5%) and may be considered a better choice for identification of product adulteration when high sensitivity levels are required. (c) 2021 Elsevier Ltd. All rights reserved. (AU)

• FAPESP's process: 18/19452-2 - Multi user equipment approved in grant 2017/50.339-5: nanoespectrofotômetro BioDrop
• Grantee: Renata Helena Branco Arnandes
• Support Opportunities: Multi-user Equipment Program