Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Molecular network for accessing polyketide derivatives from Phomopsis sp., an endophytic fungus of Casearia arborea (Salicaceae)

Full text
Author(s):
Santos, Augusto L. [1] ; Ionta, Marisa [2] ; Horvath, Renato [2] ; Soares, Marisi G. [3] ; Medeiros, Livia S. de [1] ; Uemi, Miriam [1] ; Kawafune, Eunizinis S. [4] ; Tangerina, Marcelo M. P. [4] ; Ferreira, Marcelo J. P. [4] ; Sartorelli, Patricia [1]
Total Authors: 10
Affiliation:
[1] Univ Fed Sao Paulo, Inst Environm Chem & Pharmaceut Sci, BR-09972270 Sao Paulo - Brazil
[2] Univ Fed Alfenas, Inst Biomed Sci, BR-37130000 Alfenas, MG - Brazil
[3] Univ Fed Alfenas, Inst Chem, BR-37130000 Alfenas, MG - Brazil
[4] Univ Sao Paulo, Inst Biosci, Bot Dept, BR-05508090 Sao Paulo - Brazil
Total Affiliations: 4
Document type: Journal article
Source: PHYTOCHEMISTRY LETTERS; v. 42, p. 1-7, APR 2021.
Web of Science Citations: 0
Abstract

In the last three decades endophytes have shown to be a promising source for the discovery of new cytotoxic prototypes. Casearia genus shows various cytotoxic clerodane diterpenes, but the chemistry from the plant microbiota is still unknown. In this sense, the endophytic fungus Phomopsis sp. isolated from leaves of C. arborea was investigated. The EtOAc crude extract obtained from the microbial growth in rice was dereplicated using GNPS platform, based on the molecular networking obtained from UHPLC-HR-ESI-MS/MS spectral data. The used approach pointed towards different polyketide spectral families produced by the strain. Therefore, the extract was fractionated and two new derivatives, named dothiorelones P and Q, and five previously known compounds, cytosporones A and B and dothiorelones A and B, besides 15-acetoxydothiorelone A were isolated. The chemical structures were elucidated through 1D and 2D NMR and HR-ESI-MS data analysis. Additionally, cytotoxic potential of polyketide derivatives was evaluated on human cancer cells lines (A549, MCF-7, and HepG2). Dothiorelone P and cytosporone B showed low cytotoxic activity against tumor cell lines in the tested experimental conditions. However, further studies could be addressed to investigate antiproliferative and or antimetastatic potentials of these substances. (AU)

FAPESP's process: 14/21593-2 - Baccharis L. (Asteraceae) and endophytic fungi: secondary metabolites and biological activities
Grantee:Marcelo José Pena Ferreira
Support Opportunities: Regular Research Grants
FAPESP's process: 16/24985-4 - Study the chemical composition of fungi and plants of the Atlantic Forest aimed at antitumor, antiparasitic and antimicrobial potential
Grantee:Patricia Sartorelli
Support Opportunities: Regular Research Grants
FAPESP's process: 17/16606-6 - Actinomycetes from Brazilian islands: a metabolomic approach in bioprospecting natural products with antitumor potential
Grantee:Marcelo Marucci Pereira Tangerina
Support Opportunities: Scholarships in Brazil - Post-Doctoral