Combining 3D printing and screen-printing in miniaturized, disposable sensors with carbon paste electrodes

Author(s)

Materon, Elsa Maria ^{[1, 2]} ; Wong, Ademar ^[1] ; Gomes, Leonardo Mariano ^[3] ; Ibanez-Redin, Gisela ^[2] ; Joshi, Nirav ^[2] ; Oliveira Jr, Osvaldo N. ; Faria, Ronaldo C. ^[1]

Affiliation

^[1] Univ Fed Sao Carlos, Chem Dept, CP 676, BR-13565905 Sao Carlos, SP - Brazil

^[2] Univ Sao Paulo, Sao Carlos Inst Phys, Av Trabalhador Sao Carlense 400, BR-13566590 Sao Carlos, SP - Brazil

^[3] Univ Sao Paulo, Sao Carlos Sch Engn EESC, Dept Comp & Elect Engn, Av Trabalhador Sao Carlense 400, BR-13566590 Sao Carlos, SP - Brazil

• Document type: Journal article
• Source: JOURNAL OF MATERIALS CHEMISTRY C; v. 9, n. 17 APR 2021.
• Web of Science Citations: 0

Abstract

In this paper we report on a modular design that allows one to fabricate electrochemical sensors upon combining 3D printing and screen printing. The usefulness of the design is showcased with graphene paste electrodes (GPE) coated with magnetic microspheres used to detect the drug nimesulide (NIM), biomarkers dopamine (DOP) and uric acid (UA). Under optimized conditions, the limit of detection was 0.0023 mu mol L-1 in the range from 0.5 to 9 mu mol L-1, 0.01 mu mol L-1 within the range from 0.8 to 6 mu mol L-1, and 0.0034 mu mol L-1 from 0.7 to 10 mu mol L-1 for dopamine, nimesulide, and uric acid, respectively. The three analytes could also be detected in blood serum and artificial urine samples within approximately 20 s, which is promising for clinical applications. With the modular design, the paste can be prepared with a variety of nanomaterials, functionalized with biomolecules and magnetic particles, and then stored for later use. The working electrode may also be polished to allow for reuse. Furthermore, in contrast to standard sensors made with carbon pastes, the electrochemical sensor proposed here requires small volumes (20-200 mu L), which is crucial for drug monitoring and other biomedical applications. (AU)

• FAPESP's process: 14/23546-1 - Enhanced sensitive and selective sensors made with p-NiO nanowire/n-ZnO Nanowire heterostructures
• Grantee: Niravkumar Jitendrabhai Joshi
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 18/22214-6 - Towards a convergence of technologies: from sensing and biosensing to information visualization and machine learning for data analysis in clinical diagnosis
• Grantee: Osvaldo Novais de Oliveira Junior
• Support Opportunities: Research Projects - Thematic Grants