The Influence of Silver Nanoparticles Against Toxic Effects of Philodryas olfersii Venom

Author(s)

Proenca-Assuncao, Jaqueline De Cassia ^[1] ; Farias-de-Franca, Anna Paula ^[2] ; Tribuiani, Natalia ^[1] ; Cogo, Jose Carlos ^{[3, 4]} ; Collaco, Rita de Cassia ^[5] ; Randazzo-Moura, Priscila ^[6] ; Consonni, Silvio Roberto ^[7] ; Chaud, Marco Vinicius ^[1] ; dos Santos, Carolina Alves ^[2] ; Oshima-Franco, Yoko ^[1]

Affiliation

^[1] Univ Sorocaba UNISO, Postgrad Program Pharmaceut Sci, Sorocaba, SP - Brazil

^[2] Univ Sorocaba Uniso, Pharm Grad Course, Sorocaba, SP - Brazil

^[3] Brazil Univ, Technol & Sci Inst, Bioengn Programs, Sao Paulo - Brazil

^[4] Brazil Univ, Technol & Sci Inst, Biomed Engn Programs, Sao Paulo - Brazil

^[5] State Univ Campinas UNICAMP, Fac Med Sci, Dept Pharmacol, Campinas, SP - Brazil

^[6] Pontificia Univ Catolica Sao Paulo PUCSP, Dept Surg, Sorocaba, SP - Brazil

^[7] Univ Estadual Campinas, Inst Biol, Dept Biochem & Tissue Biol, Campinas, SP - Brazil

• Document type: Journal article
• Source: INTERNATIONAL JOURNAL OF NANOMEDICINE; v. 16, p. 3555-3564, 2021.
• Web of Science Citations: 0

Abstract

Purpose: A silver nanoparticle obtained by reducing salts with solid dispersion of curcumin (130 nm, 0.081 mg mL(-1)) was used to counteract against the toxic - edematogenic, myotoxic, and neurotoxic - effects of Philodryas olfersii venom. Methods: The edematogenic effect was evaluated by plasma extravasation in rat dorsal skin after injection of 50 mu g per site of venom alone or preincubated with 1, 10, and 100 mu L of AgNPs; the myotoxicity was evaluated by measuring the creatine kinase released into the organ-bath before the treatment and at the end of each experiment; and neurotoxicity was evaluated in chick biventer cervicis using the conventional myographic technique, face to the exogenous acetylcholine (ACh) and potassium chloride (KCl) added into the bath before the treatment and after each experiment. Preliminarily, a concentration-response curve of AgNPs was carried out to select the concentration to be used for neutralizing assays, which consists of neutralizing the venom-induced neuromuscular paralysis and edema by preincubating AgNPs with venom for 30 min. Results: The P. olfersii venom-induced edema (n=6) and a complete neuromuscular blockade (n=4) that includes the total and unrecovered block of ACh and KCl contractures. AgNPs produced a concentration-dependent decrease the venom-induced edema (n=6) from 223.3% to 134.4% and to 100.5% after 10 and 100 mu L AgNPs-preincubation, respectively. The preincubation of venom with AgNPs (1 mu L; n=6) was able to maintain 46.5 +/- 10.9% of neuromuscular response under indirect stimuli, 39.2 +/- 9.7% of extrinsic nicotinic receptors functioning in absence of electrical stimulus and 28.3 +/- 8.1% of responsiveness to potassium on the sarcolemmal membrane. The CK release was not affected by any experimental protocol which was like control. Conclusion: AgNPs interact with constituents of P. olfersii venom responsible for the edema-forming activity and neuromuscular blockade, but not on the sarcolemma membrane-acting constituents. The protective effect of the studied AgNPs on avian preparation points out to molecular targets as intrinsic and extrinsic nicotinic receptors. (AU)

• FAPESP's process: 12/08271-0 - IDENTIFICATION AND PHARMACODYNAMIC OF NEUROFACILITATING FRACTIONS OF Casearia sylvestris SW. AT THE NEUROMUSCULAR JUNCTION
• Grantee: Yoko Oshima Franco
• Support Opportunities: Regular Research Grants
• FAPESP's process: 19/05891-7 - Influence of silver nanoparticles (AgNPs) on the in vitro neuromuscular blockade of Philodryas olfersii (Dipsadidae) snake venom
• Grantee: Anna Paula Farias de França
• Support Opportunities: Scholarships in Brazil - Scientific Initiation