Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Antimicrobial Resistance Profiles and Phylogenetic Analysis ofCampylobacter jejuniStrains Isolated in Brazil by Whole Genome Sequencing

Full text
Author(s):
Frazao, Miliane Rodrigues [1] ; Cao, Guojie [2] ; Medeiros, Marta Ines Cazentini [3] ; Duque, Sheila da Silva [4] ; Allard, Marc William [2] ; Falcao, Juliana Pfrimer [1]
Total Authors: 6
Affiliation:
[1] Univ Sao Paulo, FCFRP, Dept Anal Clin Toxicol & Bromatol, Ave Cafe S-N Bloco S Sala 41, BR-14040903 Ribeirao Preto, SP - Brazil
[2] US FDA, Div Microbiol, Off Regular Sci, Ctr Food Safety & Appl Nutr, College Pk, MD 20740 - USA
[3] Inst Adolfo Lutz Ribeirao Preto, Ribeirao Preto - Brazil
[4] Fundacao Oswaldo Cruz Fiocruz, Inst Oswaldo Cruz, Rio De Janeiro - Brazil
Total Affiliations: 4
Document type: Journal article
Source: MICROBIAL DRUG RESISTANCE; v. 27, n. 5, p. 660-669, MAY 1 2021.
Web of Science Citations: 1
Abstract

Aims:The objectives of this work were to use whole genome sequencing (WGS) to determine the antimicrobial resistance genotypes of 116Campylobacter jejunistrains isolated in Brazil and to compare it with the results obtained by antimicrobial susceptibility testing (AST). In addition, WGS was used to uncover the phylogenetic relationship among those strains. Results:By AST, theC. jejunistrains resistant to ciprofloxacin, tetracycline, doxycycline, and erythromycin were 51 (44%), 41 (35.3%), 41 (35.3%), and 6 (5.2%), respectively. By WGS, the genesaph(3 `)III,aadE,bla(OXA-449),bla(OXA-184),bla(OXA-61), andtet(O) were detected in 6 (5.2%), 3 (2.6%), 1 (0.9%), 10 (8.6%), 55 (47.4%), and 44 (38%) strains, respectively. Fifty-four (46.6%) strains showed the mutation T86I in thegyrAgene, and four (3.4%) strains presented the mutation A2075G in the 23S rRNA gene. The correlation between AST and WGS was 100% for ciprofloxacin, 97.5% for tetracyclines, and 66.7% for erythromycin. The whole genome single nucleotide polymorphism (SNP) tree clustered theC. jejunistrains into two clades comprising strains that were highly related from different sources, places, and years. Conclusion:The high rates ofC. jejunistrains resistant to ciprofloxacin and tetracyclines are of concern and may represent a public health problem. WGS has a potential to be a powerful tool for the prediction of resistance of antibiotics used to treat campylobacteriosis. The results obtained by whole genome SNP analysis suggested the potential for transmission between clinical and nonclinical sources and between human and animal sources over the course of 20 years in Brazil. (AU)

FAPESP's process: 14/13029-0 - Molecular characterization of Campylobacter jejuni and Campylobacter coli strains of diverse origins
Grantee:Juliana Pfrimer Falcão
Support Opportunities: Regular Research Grants
FAPESP's process: 16/24716-3 - Sequencing of the Genome, transcriptoma and phenotypic analysis of Campylobacter coli strains isolated from different sources in Brazil
Grantee:Juliana Pfrimer Falcão
Support Opportunities: Regular Research Grants
FAPESP's process: 18/06904-2 - Comparative analysis of the whole genome, transcriptome and phenotypic characterization of Campylobacter jejuni strains isolated from diverse sources during 20 years in Brazil
Grantee:Miliane Rodrigues Frazão
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 19/19338-8 - Genomic, transcriptomic and phenotypic characterization of Campylobacter jejuni strains isolated from diverse source during 20 years in Brazil
Grantee:Juliana Pfrimer Falcão
Support Opportunities: Regular Research Grants