Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Recombinant chimeric enzymes for lignocellulosic biomass hydrolysis

Full text
Author(s):
Martins, Manoela [1] ; Dinamarco, Taisa Magnani [2] ; Goldbeck, Rosana [1]
Total Authors: 3
Affiliation:
[1] Univ Estadual Campinas, Fac Food Engn, Dept Food Engn, Bioproc & Metab Engn Lab, Campinas, SP - Brazil
[2] Univ Sao Paulo, Fac Philosophy Sci & Letters Ribeirao Preto, Dept Chem, Lab Prot Biotechnol, Ribeirao Preto, SP - Brazil
Total Affiliations: 2
Document type: Review article
Source: Enzyme and Microbial Technology; v. 140, OCT 2020.
Web of Science Citations: 2
Abstract

Chimeras with improved properties can be designed by synergistically incorporating multiple proteins into one peptide chain. Such design enhances hydrolysis efficiency and lowers enzyme production costs for numerous applications, particularly in the fields of lignocellulosic biomass hydrolysis, plant-based industries, food additives, waste treatment, and biofuels. In this minireview, we summarize a brief, common approach to construct recombinant enzymes, especially in the Pichia pastoris host. We present the design strategies and the desired properties of chimeras and provide examples of recent studies in the area of lignocellulosic biomass hydrolysis. Subsequently, we describe the progress in structure prediction tools that facilitate the design of chimeric proteins toward identifying the best combinations of fusion partners and linkers. (AU)

FAPESP's process: 19/08542-3 - Enzymatic biotechnology for the xylo-oligosaccharides production from lignocellulosic agroindustrial residues aimed the production of symbiotics
Grantee:Rosana Goldbeck Coelho
Support Opportunities: Regular Research Grants