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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Meta-omics analysis indicates the saliva microbiome and its proteins associated with the prognosis of oral cancer patients

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Granato, Daniela C. [1] ; Neves, Leandro X. [1] ; Trino, Luciana D. [1] ; Carnielli, Carolina M. [1] ; Lopes, Ariane F. B. [1] ; Yokoo, Sami [1] ; Pauletti, Bianca A. [1] ; Domingues, Romenia R. [1] ; Sa, Jamile O. [1] ; Persinoti, Gabriella [1] ; Paixao, Douglas A. A. [2] ; Rivera, Cesar [1, 3] ; de Sa Patroni, Fabio M. [4] ; Tommazetto, Geizecler [5] ; Santos-Silva, Alan R. [6] ; Lopes, Marcio A. [6] ; de Castro Jr, Gilberto ; Brandao, Thais B. [7] ; Prado-Ribeiro, Ana Carolina [7] ; Squina, Fabio M. [8] ; Telles, Guilherme P. [9] ; Paes Leme, Adriana F. [1]
Total Authors: 22
Affiliation:
[1] CNPEM, Lab Nacl Biociencias, LNBio, Campinas, SP - Brazil
[2] CNPEM, Lab Nacl Biorrenovaveis, LNBr, Campinas, SP - Brazil
[3] Univ Talca, Fac Ciencias Salud, Dept Estomatol, Talca, Region Del Maul - Chile
[4] Univ Estadual Campinas, Ctr Biol Mol & Engn Genet, Campinas, SP - Brazil
[5] Aarhus Univ, Dept Biol & Chem Engn BCE, DK-82000 Aarhus - Denmark
[6] Univ Estadual Campinas UNICAMP, Fac Odontol Piracicaba, Dept Diagnost Oral, Piracicaba, SP - Brazil
[7] Inst Canc Estado DesaoPaulo, Octavio Friers Oliveira, Sao Paulo - Brazil
[8] Univ Sorocaba, Dept Proc Tecnol & Ambientais, Sao Paulo - Brazil
[9] Univ Estadual Campinas, Inst Comp, Campinas, SP - Brazil
Total Affiliations: 9
Document type: Journal article
Source: BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS; v. 1869, n. 8 AUG 2021.
Web of Science Citations: 0
Abstract

Saliva is a biofluid that maintains the health of oral tissues and the homeostasis of oral microbiota. Studies have demonstrated that Oral squamous cell carcinoma (OSCC) patients have different salivary microbiota than healthy individuals. However, the relationship between these microbial differences and clinicopathological outcomes is still far from conclusive. Herein, we investigate the capability of using metagenomic and metaproteomic saliva profiles to distinguish between Control (C), OSCC without active lesion (L0), and OSCC with active lesion (L1) patients. The results show that there are significantly distinct taxonomies and functional changes in L1 patients compared to C and L0 patients, suggesting compositional modulation of the oral microbiome, as the relative abundances of Centipeda, Veillonella, and Gemella suggested by metagenomics are correlated with tumor size, clinical stage, and active lesion. Metagenomics results also demonstrated that poor overall patient survival is associated with a higher relative abundance of Stenophotromonas, Staphylococcus, Centipeda, Selenomonas, Alloscordovia, and Acitenobacter. Finally, compositional and functional differences in the saliva content by metaproteomics analysis can distinguish healthy individuals from OSCC patients. In summary, our study suggests that oral microbiota and their protein abundance have potential diagnosis and prognosis value for oral cancer patients. Further studies are necessary to understand the role of uniquely detected metaproteins in the microbiota of healthy and OSCC patients as well as the crosstalk between saliva host proteins and the oral microbiome present in OSCC. (AU)

FAPESP's process: 18/18496-6 - The role of alcohol treated-extracellular vesicles in oral cells transformation
Grantee:Adriana Franco Paes Leme
Support type: Research Projects - Thematic Grants
FAPESP's process: 18/02180-0 - Study of the glycoprotein composition in the surface of extracellular vesicles of plasma from patients with oral cancer and its correlation to prognosis
Grantee:Carolina Moretto Carnielli
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 16/07846-0 - Peptidomic analysis of extracelular vesicles originated from cell lines, saliva and plasma from oral squamous cell carcinoma
Grantee:Adriana Franco Paes Leme
Support type: Regular Research Grants
FAPESP's process: 19/18751-9 - Discovery and validation of prognostic candidates for oral cancer in liquid biopsies.
Grantee:Daniela Campos Granato
Support type: Scholarships in Brazil - Young Researchers
FAPESP's process: 19/21815-9 - Multiplex N-terminomics analysis of cardiomyocyte secretome based on MMP-2 and ADAM17 activity using TMT-TAILS quantitative proteomics
Grantee:Ariane Fidelis Busso Lopes
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 09/54067-3 - Acquisition of a mass spectrometer coupled to a liquid chromatography system for increasing the capacity to meet the needs of users and for making new technologies available in the Laboratory of Mass Spectrometry
Grantee:Adriana Franco Paes Leme
Support type: Multi-user Equipment Program
FAPESP's process: 18/11958-4 - Proteomic Analysis of Extracellular Vesicles from Saliva and Plasma of Patients with Squamous Cells Carcinoma and the Study of Post-Translational Modifications and Identification of Disease Markers
Grantee:Leandro Xavier Neves
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 15/50590-4 - Lignin valorization in cellulosic ethanol plants: biocatalytic conversion via ferulic acid to high value chemicals
Grantee:Fábio Márcio Squina
Support type: Program for Research on Bioenergy (BIOEN) - Thematic Grants
FAPESP's process: 18/12194-8 - Immobilization of multiple potential biomarkers, pure and obtained from liquid biopsies, in Metal-Organic Frameworks (MOFs) as biosensors applied in head and neck Cancer diagnosis and prognosis
Grantee:Luciana Daniele Trino Albano
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 18/15728-3 - Association of extracellular vesicles derived from fibroblasts with the progression of Oral Squamous Cell Carcinoma
Grantee:Jamile de Oliveira Sá
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 14/06485-9 - Investigation of the ADAM17 metalloproteinase regulation mechanism by the interaction with Trx-1
Grantee:Rute Alves Pereira e Costa
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 18/15535-0 - From discovery to validation of prognostic candidates for oral cancer in liquid biopsies
Grantee:Daniela Campos Granato
Support type: Research Grants - Young Investigators Grants
FAPESP's process: 10/19278-0 - Study of regulation of ADAMs in oral cancer
Grantee:Adriana Franco Paes Leme
Support type: Research Grants - Young Investigators Grants