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Immobilization of multiple potential biomarkers, pure and obtained from liquid biopsies, in Metal-Organic Frameworks (MOFs) as biosensors applied in head and neck Cancer diagnosis and prognosis

Grant number: 18/12194-8
Support Opportunities:Scholarships in Brazil - Post-Doctoral
Effective date (Start): September 01, 2018
Effective date (End): April 30, 2024
Field of knowledge:Health Sciences - Dentistry - Dental Clinics
Principal Investigator:Adriana Franco Paes Leme
Grantee:Luciana Daniele Trino Albano
Host Institution: Centro Nacional de Pesquisa em Energia e Materiais (CNPEM). Ministério da Ciência, Tecnologia e Inovações (Brasil). Campinas , SP, Brazil
Associated research grant:18/18496-6 - The role of alcohol treated-extracellular vesicles in oral cells transformation, AP.TEM
Associated scholarship(s):21/14880-9 - Nanoscale metal-organic frameworks for drug delivery to oral cancer, BE.EP.PD


Among the different types of tumors, head and neck Cancer is the sixth most common form of malignant tumor, and early diagnosis of the disease is a decisive factor in controlling its progression. In this way, the immobilization of biomarkers for head and neck Cancer in nanomaterials arises as a powerful tool for the diagnosis and prognosis of the disease, allowing a greater success in the treatment and improvement in the patients' survival. Among the types of nanomaterials, the Metal-Organic Frameworks (MOFs) are an attractive class because they present several active sites. Therefore, this proposal aims to immobilize simultaneously multiple biomarkers candidates of head and neck Cancer and proteins from liquid biopsies (saliva) in metal-organic frameworks, ZIF-8 and NMOF-1, to develop biosensors for diagnosis and prognosis of the disease. The potential biomarkers, previously verified from our group in saliva of patients with oral Cancer that will be initially used are C3, CFB, C4B, SERPINA1 and LRG1. The immobilization of the biomarkers in MOFs will be evaluated through different strategies of antibody antigen interactions to improve its specificity. The biosensors will be characterized by electrochemical or spectrophotometric method. In addition, saliva samples will be analyzed against the response of the biosensors. As proof of concept, a biosensor sensitivity and specificity comparison will be made with ELISA assays and mass spectrometry analysis. This proposal can provide a significant advance in the diagnosis and prognosis of Cancer, since to the best of our knowledge no strategy of this type, specific for head and neck Cancer using multiple biomarkers or biological samples, has been found in the literature. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
E COSTA, RUTE A. P.; GRANATO, DANIELA C.; TRINO, LUCIANA D.; YOKOO, SAMI; CARNIELLI, CAROLINA M.; KAWAHARA, REBECA; DOMINGUES, ROMENIA R.; PAULETTI, BIANCA ALVES; NEVES, LEANDRO XAVIER; SANTANA, ALINE G.; et al. ADAM17 cytoplasmic domain modulates Thioredoxin-1 conformation and activity. REDOX BIOLOGY, v. 37, . (16/01528-7, 18/12194-8, 19/18751-9, 18/18496-6, 09/54067-3, 18/15535-0, 14/23888-0, 16/07846-0, 16/24664-3, 14/06485-9, 10/19278-0)
GRANATO, DANIELA C.; NEVES, LEANDRO X.; TRINO, LUCIANA D.; CARNIELLI, CAROLINA M.; LOPES, ARIANE F. B.; YOKOO, SAMI; PAULETTI, BIANCA A.; DOMINGUES, ROMENIA R.; SA, JAMILE O.; PERSINOTI, GABRIELLA; et al. Meta-omics analysis indicates the saliva microbiome and its proteins associated with the prognosis of oral cancer patients. BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, v. 1869, n. 8, . (18/18496-6, 18/02180-0, 16/07846-0, 19/18751-9, 19/21815-9, 09/54067-3, 18/11958-4, 15/50590-4, 18/12194-8, 18/15728-3, 14/06485-9, 18/15535-0, 10/19278-0)
SA, JAMILE DE OLIVEIRA; TRINO, LUCIANA DANIELE; OLIVEIRA, ANA KARINA; BUSSO LOPES, ARIANE FIDELIS; GRANATO, DANIELA CAMPOS; COSTA NORMANDO, ANA GABRIELA; SANTOS, ERISON SANTANA; NEVES, LEANDRO XAVIER; CARNIELLI, CAROLINA MORETTO; PAES LEME, ADRIANA FRANCO. Proteomic approaches to assist in diagnosis and prognosis of oral cancer. EXPERT REVIEW OF PROTEOMICS, v. 18, n. 4, p. 261-284, . (18/11958-4, 19/21815-9, 18/18496-6, 18/12194-8, 19/18751-9, 19/09692-9, 18/02180-0, 18/15535-0, 18/15728-3, 16/19337-3)

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