Meta-omics analysis indicates the saliva microbiome and its proteins associated with the prognosis of oral cancer patients

Author(s):Show less -

Granato, Daniela C. ^[1] ; Neves, Leandro X. ^[1] ; Trino, Luciana D. ^[1] ; Carnielli, Carolina M. ^[1] ; Lopes, Ariane F. B. ^[1] ; Yokoo, Sami ^[1] ; Pauletti, Bianca A. ^[1] ; Domingues, Romenia R. ^[1] ; Sa, Jamile O. ^[1] ; Persinoti, Gabriella ^[1] ; Paixao, Douglas A. A. ^[2] ; Rivera, Cesar ^{[1, 3]} ; de Sa Patroni, Fabio M. ^[4] ; Tommazetto, Geizecler ^[5] ; Santos-Silva, Alan R. ^[6] ; Lopes, Marcio A. ^[6] ; de Castro Jr, Gilberto ; Brandao, Thais B. ^[7] ; Prado-Ribeiro, Ana Carolina ^[7] ; Squina, Fabio M. ^[8] ; Telles, Guilherme P. ^[9] ; Paes Leme, Adriana F. ^[1]

Affiliation

^[1] CNPEM, Lab Nacl Biociencias, LNBio, Campinas, SP - Brazil

^[2] CNPEM, Lab Nacl Biorrenovaveis, LNBr, Campinas, SP - Brazil

^[3] Univ Talca, Fac Ciencias Salud, Dept Estomatol, Talca, Region Del Maul - Chile

^[4] Univ Estadual Campinas, Ctr Biol Mol & Engn Genet, Campinas, SP - Brazil

^[5] Aarhus Univ, Dept Biol & Chem Engn BCE, DK-82000 Aarhus - Denmark

^[6] Univ Estadual Campinas UNICAMP, Fac Odontol Piracicaba, Dept Diagnost Oral, Piracicaba, SP - Brazil

^[7] Inst Canc Estado DesaoPaulo, Octavio Friers Oliveira, Sao Paulo - Brazil

^[8] Univ Sorocaba, Dept Proc Tecnol & Ambientais, Sao Paulo - Brazil

^[9] Univ Estadual Campinas, Inst Comp, Campinas, SP - Brazil

• Document type: Journal article
• Source: BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS; v. 1869, n. 8 AUG 2021.
• Web of Science Citations: 0

Abstract

Saliva is a biofluid that maintains the health of oral tissues and the homeostasis of oral microbiota. Studies have demonstrated that Oral squamous cell carcinoma (OSCC) patients have different salivary microbiota than healthy individuals. However, the relationship between these microbial differences and clinicopathological outcomes is still far from conclusive. Herein, we investigate the capability of using metagenomic and metaproteomic saliva profiles to distinguish between Control (C), OSCC without active lesion (L0), and OSCC with active lesion (L1) patients. The results show that there are significantly distinct taxonomies and functional changes in L1 patients compared to C and L0 patients, suggesting compositional modulation of the oral microbiome, as the relative abundances of Centipeda, Veillonella, and Gemella suggested by metagenomics are correlated with tumor size, clinical stage, and active lesion. Metagenomics results also demonstrated that poor overall patient survival is associated with a higher relative abundance of Stenophotromonas, Staphylococcus, Centipeda, Selenomonas, Alloscordovia, and Acitenobacter. Finally, compositional and functional differences in the saliva content by metaproteomics analysis can distinguish healthy individuals from OSCC patients. In summary, our study suggests that oral microbiota and their protein abundance have potential diagnosis and prognosis value for oral cancer patients. Further studies are necessary to understand the role of uniquely detected metaproteins in the microbiota of healthy and OSCC patients as well as the crosstalk between saliva host proteins and the oral microbiome present in OSCC. (AU)

• FAPESP's process: 18/18496-6 - The role of alcohol treated-extracellular vesicles in oral cells transformation
• Grantee: Adriana Franco Paes Leme
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 18/02180-0 - Study of the glycoprotein composition in the surface of extracellular vesicles of plasma from patients with oral cancer and its correlation to prognosis
• Grantee: Carolina Moretto Carnielli
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 16/07846-0 - Peptidomic analysis of extracelular vesicles originated from cell lines, saliva and plasma from oral squamous cell carcinoma
• Grantee: Adriana Franco Paes Leme
• Support Opportunities: Regular Research Grants
• FAPESP's process: 19/18751-9 - Discovery and validation of prognostic candidates for oral cancer in liquid biopsies.
• Grantee: Daniela Campos Granato
• Support Opportunities: Scholarships in Brazil - Young Researchers
• FAPESP's process: 19/21815-9 - Multiplex N-terminomics analysis of cardiomyocyte secretome based on MMP-2 and ADAM17 activity using TMT-TAILS quantitative proteomics
• Grantee: Ariane Fidelis Busso Lopes
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 09/54067-3 - Acquisition of a mass spectrometer coupled to a liquid chromatography system for increasing the capacity to meet the needs of users and for making new technologies available in the Laboratory of Mass Spectrometry
• Grantee: Adriana Franco Paes Leme
• Support Opportunities: Multi-user Equipment Program
• FAPESP's process: 18/11958-4 - Proteomic Analysis of Extracellular Vesicles from Saliva and Plasma of Patients with Squamous Cells Carcinoma and the Study of Post-Translational Modifications and Identification of Disease Markers
• Grantee: Leandro Xavier Neves
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 15/50590-4 - Lignin valorization in cellulosic ethanol plants: biocatalytic conversion via ferulic acid to high value chemicals
• Grantee: Fábio Márcio Squina
• Support Opportunities: Program for Research on Bioenergy (BIOEN) - Thematic Grants
• FAPESP's process: 18/12194-8 - Immobilization of multiple potential biomarkers, pure and obtained from liquid biopsies, in Metal-Organic Frameworks (MOFs) as biosensors applied in head and neck Cancer diagnosis and prognosis
• Grantee: Luciana Daniele Trino Albano
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 18/15728-3 - Association of extracellular vesicles derived from fibroblasts with the progression of Oral Squamous Cell Carcinoma
• Grantee: Jamile de Oliveira Sá
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 14/06485-9 - Investigation of the ADAM17 metalloproteinase regulation mechanism by the interaction with Trx-1
• Grantee: Rute Alves Pereira e Costa
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 18/15535-0 - From discovery to validation of prognostic candidates for oral cancer in liquid biopsies
• Grantee: Daniela Campos Granato
• Support Opportunities: Research Grants - Young Investigators Grants
• FAPESP's process: 10/19278-0 - Study of regulation of ADAMs in oral cancer
• Grantee: Adriana Franco Paes Leme
• Support Opportunities: Research Grants - Young Investigators Grants