Evaluation of the optimum dietary essential amino acid pattern for adult pacu (Piaractus mesopotamicus)

Author(s)

Oliveira, Thais S. ^[1] ; Khan, Kifayat U. ^[1] ; Boaratti, Andre Z. ^[1] ; Rodrigues, Andressa T. ^[1] ; Reis, Matheus P. ^[2] ; Sakomura, Nilva K. ^[2] ; Fernandes, Joao B. K. ^[1]

Affiliation

^[1] Sao Paulo State Univ UNESP, Aquaculture Ctr, Via Acesso Prof Paulo Donato Castelane, Vila Ind, BR-14884900 Jaboticabal, SP - Brazil

^[2] Sao Paulo State Univ UNESP, Fac Agr & Vet Sci, Dept Anim Sci, BR-14884900 Jaboticabal, SP - Brazil

• Document type: Journal article
• Source: Aquaculture; v. 540, JUL 15 2021.
• Web of Science Citations: 1

Abstract

The optimum levels of essential amino acids (EAAs) are required to be maintained in fish feed for obtaining normal body protein synthesis and muscle growth. This study aimed to determine the optimum dietary EAA pattern for adult pacu (Piaractus mesopotamicus) by the amino acid (AA) deletion method. A total of 165 adult fish of 1109.83 +/- 14.26 g (initial body weight +/- standard deviation) were equally distributed among 11 treatments in a recirculation aquaculture system. Each of the 11 treatments consisted of three randomly arranged replicates (tanks) and 5 fish were stocked per tank. During the 60-day experimental period, fish were fed with 11 diets including a control diet (CD) and 10 EAA limiting diets (LDs) two times daily until apparent satiation. The body nitrogen (N) retention and weight gain in fish fed with EAA LDs was lower (P 0.05) than the CD. The feed efficiency in fish fed with CD was similar (P 0.05) to most EAA LDs, except arginine and histidine LDs. The specific growth rate in fish fed with tryptophan, arginine and histidine LDs was different (P < 0.05) as compared to the CD. Based on the present N retention data obtained through the AA deletion method, the optimum pattern of dietary EAAs estimated in relation to lysine requirement (100%) was as follows: arginine 72.16%; phenylalanine 40.83%; histidine 30.15%; isoleucine 53.01%; leucine 81.71%; methionine 24.97%; threonine 58.20%; tryptophan 9.13% and valine 54.69%. (AU)

• FAPESP's process: 19/06343-3 - Optimal essential amino acids ratio for pacu in the termination phase by the deletion method
• Grantee: Thaís da Silva Oliveira
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 13/25761-4 - Modeling production and nutritional requirements of poultry and fish
• Grantee: Nilva Kazue Sakomura
• Support Opportunities: Research Projects - Thematic Grants