A probiotic has differential effects on allergic airway inflammation in A/J and C57BL/6 mice and is correlated with the gut microbiome

Author(s):Show less -

Casaro, Mateus B. ^[1] ; Thomas, Andrew M. ^{[2, 3, 4]} ; Mendes, Eduardo ^[1] ; Fukumori, Claudio ^[1] ; Ribeiro, Willian R. ^[1] ; Oliveira, Fernando A. ^[5] ; Crisma, Amanda R. ^[6] ; Murata, Gilson M. ^[7] ; Bizzarro, Bruna ^[8] ; Sa-Nunes, Anderson ^[8] ; Setubal, Joao C. ^[2] ; Mayer, Marcia P. A. ^[9] ; Martins, Flaviano S. ^[10] ; Vieira, Angelica T. ^[11] ; Antiorio, Ana T. F. B. ^[12] ; Tavares-de-Lima, Wothan ^[13] ; Camara, Niels O. S. ^[8] ; Curi, Rui ^[14] ; Dias-Neto, Emmanuel ^{[4, 15]} ; Ferreira, Caroline M. ^[1]

Affiliation:Show less -

^[1] Univ Fed Sao Paulo, Inst Environm Chem & Pharmaceut Sci, Dept Pharmaceut Sci, R Sao Nicolau 210, BR-0991303 Diadema, SP - Brazil

^[2] Univ Sao Paulo, Inst Chem, Dept Biochem, Sao Paulo - Brazil

^[3] Univ Trento, Dept CIBIO, Trento - Italy

^[4] CIPE AC Camargo Canc Ctr, Med Genom Lab, Sao Paulo - Brazil

^[5] Fed Univ ABC UFABC, Ctr Math Comp & Cognit CMCC, Sao Bernardo Do Campo, SP - Brazil

^[6] Univ Fed Parana, Dept Clin Anal, Curitiba, Parana - Brazil

^[7] Univ Sao Paulo, Dept Clin Med, Fac Med, BR-01246903 Sao Paulo - Brazil

^[8] Univ Sao Paulo, Inst Biomed Sci, Dept Immunol, Sao Paulo - Brazil

^[9] Univ Sao Paulo, Inst Biomed Sci, Dept Microbiol, Sao Paulo, SP - Brazil

^[10] Univ Fed Minas Gerais, Inst Biol Sci, Dept Microbiol, Belo Horizonte, MG - Brazil

^[11] Univ Fed Minas Gerais, Biol Sci Inst, Dept Biochem & Immunol, Belo Horizonte, MG - Brazil

^[12] Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Pathol, Sao Paulo - Brazil

^[13] Univ Sao Paulo, Inst Biomed Sci 1, Dept Pharmacol, Sao Paulo - Brazil

^[14] Univ Cruzeiro Sul, Interdisciplinary Post Grad Program Hlth Sci, Sao Paulo - Brazil

^[15] Univ Sao Paulo, Inst Psychiat, Sch Med, Lab Neurosci LIM 27, Sao Paulo - Brazil

• Document type: Journal article
• Source: MICROBIOME; v. 9, n. 1 JUN 10 2021.
• Web of Science Citations: 1

Abstract

The phenotypes of allergic airway diseases are influenced by the interplay between host genetics and the gut microbiota, which may be modulated by probiotics. We investigated the probiotic effects on allergic inflammation in NJ and C57BU6 mice. C57BU6 mice had increased gut microbiota diversity compared to NJ mice at baseline. Acetate producer probiotics differentially modulated and altered the genus abundance of specific bacteria, such as Akkermansia and Allistipes, in mouse strains. We induced airway inflammation followed by probiotic treatment and found that only A/J mice exhibited decreased inflammation, and the beneficial effects of probiotics in NJ mice were partially due to acetate production. To understand the relevance of microbial composition colonization in the development of allergic diseases, we implanted female C57BU6 mice with NJ embryos to naturally modulate the microbial composition of NJ mice, which increased gut microbiota diversity and reduced eosinophilic inflammation in A/J. These data demonstrate the central importance of microbiota to allergic phenotype severity. (AU)

• FAPESP's process: 12/50410-8 - Effects of short-chain fatty acids produced by probiotic bacteria in the prophylaxis and treatment of allergic airway inflammation
• Grantee: Caroline Marcantonio Ferreira
• Support Opportunities: Research Grants - Young Investigators Grants
• FAPESP's process: 16/13496-2 - Symbiotic administration's role in the perinatal period in experimental allergic asthma in offspring
• Grantee: Mateus Campos Casaro
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 19/12324-1 - Probiotic as a therapeutic tool in the skin-lung-bowel axis
• Grantee: Caroline Marcantonio Ferreira
• Support Opportunities: Regular Research Grants